Radioluminography for quantitative autoradiography of 14C

European Journal of Drug Metabolism and Pharmacokinetics
N MotojiA Shigematsu

Abstract

The aim of this study was to elucidate whether or not quantitative analysis of autoradiographs could be obtained with a [14C]-labelled compound by the use of new type radiosensor, so called 'imaging plate' (IP). Since X-ray films were first used as a radiosensor for macroautoradiography by Ullberg (1), many kinds of X-ray films have been used but the other sensors have never been used. Our results indicated that there was an excellent relation between relative intensity (PSL-BG) and radioactivity on a given area which was completely linear. The linearity was observed in a relatively wide range between 10(1)-10(5) dpm orders of radioactivity. About 100 times greater sensitivity of the IP than any X-ray film was demonstrated by the use of not only [14C]-radioactive standard sources but also by experimentally provided [14C)-radioactive spots developed on a TLC plate and macroautoradiographs (MARG). In order to obtain reliable quantitative data from a MARG image, it was required that a thin specimen section be kept at a constant level of thickness under freezing condition through out the exposure time. Results also showed that the linear relation of relative intensity versus radioactivity of the specimens had a very wide range from...Continue Reading

Citations

Nov 24, 2007·Archives of Toxicology·Veronica LindströmOrjan Lindhe
May 25, 2012·Drug and Chemical Toxicology·María Luisa Lucero, Andrew B Patterson
Dec 20, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·L MartínezS Puig
May 12, 2000·Regulatory Toxicology and Pharmacology : RTP·U BuschG Nehmiz
Mar 17, 2004·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Merja HaaparantaOlof Solin
Jan 13, 2000·FEBS Letters·T MoriT Okamoto
Aug 6, 2019·Journal of Labelled Compounds & Radiopharmaceuticals·Simona MarzoratiAlberto Ghiglieri

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