Radioprotective properties of apple polyphenols: an in vitro study

Molecular and Cellular Biochemistry
Pankaj ChaudharyRakesh Kumar Sharma

Abstract

Present study was undertaken to evaluate the radioprotective ability of total polyphenols extracted from edible portion (epicarp and mesocarp) of apple. Prior administration of apple polyphenols to murine thymocytes significantly countered radiation induced DNA damage (evaluated by alkaline halo assay) and cell death (trypan blue exclusion method) in a dose dependent manner maximally at a concentration of 2 and 0.2 mg/ml respectively. Apple polyphenols in a dose dependent fashion inhibited both radiation or Fenton reaction mediated 2-deoxyribose (2-DR) degradation indicating its ability to scavenge hydroxyl radicals and this activity was found to be unaltered in presence of simulated gastric juice. Similarly apple polyphenols in a dose dependent fashion scavenged DPPH radicals (maximum 69% at 1 mg/ml), superoxide anions (maximum 88% at 2 mg/ml), reduced Fe(3 +) to Fe(2 +) (maximum at 1 mg/ml) and inhibited Fenton reaction mediated lipid peroxidation (maximum 66% at 1.5 mg/ml) further establishing its antioxidative properties. Studies carried out with plasmid DNA revealed the ability of apple polyphenols to inhibit radiation induced single as well as double strand breaks. The results clearly indicate that apple polyphenols have ...Continue Reading

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Citations

Jul 8, 2009·Arhiv za higijenu rada i toksikologiju·Vesna BenkovićNevenka Kopjar
Mar 23, 2013·Radiation and Environmental Biophysics·Sandip PalSubrata Kumar Dey
Jun 2, 2009·Toxicology and Industrial Health·E KadiogluA E Karakaya
Nov 22, 2013·Redox Report : Communications in Free Radical Research·Halina Malgorzata ZbikowskaPaweł Nowak
Sep 2, 2008·Biological & Pharmaceutical Bulletin·Vesna BenkovićNada Orolić
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shadab FaramarziSeverina Pacifico

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