Radioresistant Sf9 insect cells display moderate resistance against cumene hydroperoxide.

Molecular and Cellular Biochemistry
Jyoti Swaroop KumarSudhir Chandna

Abstract

Lepidopteran insect cells serve as excellent model to study stress responses and are known to display resistance against DNA damaging agents including ionizing radiation; however, limited information is available on the effects of membrane damaging agents in these cells. In this study, we investigated the response of Sf9 cells (derived from ovaries of Spodoptera frugiperda; order Lepidoptera) to cumene hydroperoxide (CHPx), compared to human BMG-1 cells. CHPx treatment at doses lethal for human cells also caused typical necrosis in Sf9. Severe necrosis in human BMG-1 cells was observed at 125 μM, whereas similar effect in Sf9 cells was observed at 250 μM. In Sf9 cells, CHPx (250 μM) induced negligible changes in mitochondrial membrane potential and intracellular reactive oxygen species, while moderate effect was observed on intracellular calcium distribution. Reduced DNA damage and lipid (including cardiolipin) oxidation was observed in Sf9 cells that could be due to moderate total antioxidant status and constitutive/induced glutathione S-transferase activity. This study importantly demonstrates that Lepidopteran insect cells having extensive resistance towards DNA damaging agents show only moderately higher resistance to membr...Continue Reading

References

Sep 15, 1975·European Journal of Biochemistry·H Sies, K H Summer
Jul 1, 1992·Cardiovascular Research·M Persoon-RothertA van der Laarse
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·S Tsuchida, K Sato
Apr 1, 1990·Trends in Biochemical Sciences·J M Gutteridge, B Halliwell
Nov 1, 1988·International Journal of Radiation Biology·T M Koval, E R Kazmar
Jan 1, 1988·CRC Critical Reviews in Biochemistry·B Mannervik, U H Danielson
Feb 15, 1988·Archives of Biochemistry and Biophysics·M A Tirmenstein, D J Reed
Nov 15, 1986·Archives of Biochemistry and Biophysics·R H Weiss, R W Estabrook
Jul 1, 1985·Journal of Molecular and Cellular Cardiology·J F KosterH Stam
Jan 28, 1994·Biochemical and Biophysical Research Communications·J A Bonner, V Liengswangwong
May 31, 1996·The Journal of Biological Chemistry·T T YaminD K Miller
Jun 1, 1997·Free Radical Research·A GuidarelliO Cantoni
May 23, 2001·Free Radical Biology & Medicine·Y WangD W Murhammer
Jul 26, 2002·Toxicology and Applied Pharmacology·Yusong YangYogesh C Awasthi
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Xiuli LiuRick G Schnellmann
Jun 19, 2004·International Journal of Radiation Biology·S ChandnaV Jain
Jan 5, 2006·Genes & Development·Wei-Xing Zong, Craig B Thompson
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T M Koval
Feb 20, 2007·Biochemical and Biophysical Research Communications·Atchasai SiritantikornRalf Morgenstern
Jan 23, 2008·Biological Chemistry·Catharina Bertram, Ralf Hass
Mar 19, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Arne LindenHasso Seibert
Dec 5, 2008·Bioinformation·Shubhankar SumanSudhir Chandna
Mar 7, 2009·Mutagenesis·I-Cheng ChengT C Wang
Dec 17, 2009·Bioinformation·Shubhankar SumanSudhir Chandna
Jun 11, 2011·The International Journal of Biochemistry & Cell Biology·Shubhankar SumanSudhir Chandna
Aug 4, 2011·Journal of Biochemical and Molecular Toxicology·F VimardD Duval

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Citations

Mar 22, 2019·Scientific Reports·Bárbara Della NoceCarlos Logullo
May 23, 2015·International Journal of Radiation Biology·Shubhankar SumanRakesh Kumar Seth
Jul 19, 2013·International Journal of Radiation Biology·Sudhir ChandnaRakesh Kumar Seth

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