PMID: 2503805Apr 1, 1989Paper

Radiorespirometric detection of macrofilaricidal activity in vitro

Parasitology
J C Comley, M J Rees

Abstract

An in vitro method for studying radiorespiration has been adapted to single macrofilariae. Using this method viable (but not heat-killed) Dipetalonema viteae and Onchocera gibsoni macrofilariae evolved measurable amounts of 14CO2 from L-[U-14C]glutamine. Nonlinear and less uniform rates of 14CO2 evolution were demonstrated with D-[U-14C]glucose, [1-14C]acetate and [1-14C]octanoate. These findings led us to develop an in vitro screen in which inhibition of 14CO2 evolution from L-[U-14C]glutamine was used as a parameter for gauging macrofilaricidal activity. Using this screen we have examined the activity of 17 'so-called' antifilarial standards and found a greater degree of sensitivity than shown by other biochemical criteria. Other data obtained suggest a role for radiorespirometry in determining the viability of fragments of Onchocerca tissue.

References

Aug 1, 1977·The Journal of protozoology·J E DeckerG V Levin
Feb 1, 1989·International Journal for Parasitology·J C ComleyD C Jenkins
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·R Lainson
Dec 1, 1988·Parasitology·A M AgnewM J Doenhoff

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Citations

Jun 1, 1990·Molecular and Biochemical Parasitology·K P Davies, P Köhler
Dec 17, 2009·Journal of Experimental Botany·Wojciech StrzalkaAlicja Ziemienowicz
Feb 9, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Valya R RussanovaBruce H Howard
Jan 1, 1991·Parasitology Today·P Köhler

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