PMID: 7519736Aug 1, 1994Paper

Radiosensitive target in the early mouse embryo exposed to very low doses of ionizing radiation

Mutation Research
L M WileyT Straume

Abstract

We exposed mouse preimplantation embryos in vitro to either tritiated water (HTO) or tritiated thymidine (TdR) to determine whether the radiosensitive target was nuclear or extranuclear for embryonic cell proliferation disadvantage in the mouse embryo chimera assay. 8-cell embryos were incubated in either HTO or TdR for 2 h and paired with non-irradiated control embryos to form chimeras. Chimeras were cultured for an average of 20.2 h to allow for 2-3 cell cycles and then partially dissociated to obtain the number of progeny cells contributed by the two partner embryos for each chimera. These values were expressed as a "proliferation ratio" (number of cells from the irradiated embryo: total number of cells in the chimera). A ratio significantly less than 0.50 indicates that the experimental embryo expressed an embryonic cell proliferation disadvantage, which is the endpoint of this assay. The activity concentrations of HTO and TdR were adjusted so that both would deliver comparable mean absorbed nuclear doses during the combined initial 2-h irradiation incubation and subsequent 20.2 h chimera incubation periods. Although nuclear doses were comparable under these conditions, the extranuclear dose delivered by the uniformly distr...Continue Reading

References

May 1, 1991·Mutation Research·T StraumeR L Dobson
Oct 11, 1988·Biochimica Et Biophysica Acta·P G PlagemannC Woffendin
Jan 1, 1988·Journal of Reproduction and Fertility·K M EbertN B Hecht
Jan 1, 1986·Journal of Reproduction and Fertility·R K Smith, M H Johnson
Feb 1, 1973·Journal of Reproduction and Fertility·J W Overstreet
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W L Russell, E M Kelly
Sep 1, 1982·Journal of Reproduction and Fertility·P QuinnD G Whittingham
Dec 1, 1993·Health Physics·T Straume, A L Carsten

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