Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays

British Journal of Cancer
C S DjuzenovaM Flentje

Abstract

Spontaneous and radiation-induced genetic instability of peripheral blood mononuclear cells derived from unselected breast cancer (BC) patients (n=50) was examined using the single-cell gel electrophoresis (Comet) assay and a modified G2 micronucleus (MN) test. Cells from apparently healthy donors (n=16) and from cancer patients (n=9) with an adverse early skin reaction to radiotherapy (RT) served as references. Nonirradiated cells from the three tested groups exhibited similar baseline levels of DNA fragmentation assessed by the Comet assay. Likewise, the Comet analysis of in vitro irradiated (5 Gy) cells did not reveal any significant differences among the three groups with respect to the initial and residual DNA fragmentation, as well as the DNA repair kinetics. The G2 MN test showed that cells from cancer patients with an adverse skin reaction to RT displayed increased frequencies of both spontaneous and radiation-induced MN compared to healthy control or the group of unselected BC patients. Two patients from the latter group developed an increased early skin reaction to RT, which was associated with an increased initial DNA fragmentation in vitro only in one of them. Cells from the other BC patient exhibited a striking slo...Continue Reading

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Citations

Oct 22, 2009·Mutagenesis·Kim Vande LoockMicheline Kirsch-Volders
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May 19, 2010·Mutation Research·Ilse DecordierMicheline Kirsch-Volders

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Methods Mentioned

BETA
chromosomal aberrations
electrophoresis
density-gradient centrifugation
X-ray

Software Mentioned

Komet
Origin

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