Raf-1 kinase regulates smooth muscle contraction in the rat mesenteric arteries.

Journal of Vascular Research
Kunju SathishkumarChandra Yallampalli

Abstract

We investigated the potential role of Raf-1 kinase in mesenteric arterial contraction. Inhibitors of Raf-1 kinase, GW5074, L779450 and ZM 336372 reversed phenylephrine (PE)-induced mesenteric vascular contraction. Studies in vivo in rats showed that GW5074 inhibited PE-induced increase in mean arterial pressure in adult female Sprague-Dawley rats. Isometric tension studies in mesenteric arteries of rats showed that GW5074 did not change the KCl-evoked contraction but significantly inhibited the contractions to PE, 5-HT, U46619, endothelin 1, angiotensin II and phorbol 12, 13-dibutyrate (PDBu). In mesenteric vascular smooth muscle cells (VSMCs), PE stimulated increase in Raf-1 phosphorylation which was inhibited by GW5074. Measurement of [Ca(2+)](i) with Fura-2 showed that GW5074-mediated inhibition of PE-induced contraction was not associated with decreases in [Ca(2+)](i). VSMCs treated with PE exhibited higher levels of the contractile proteins, p-MYPT1 and p-MLC(20), which was inhibited by GW5074. Similarly, PDBu induced increases in phosphorylation of Raf-1, MLC(20) and MYPT1 and this was inhibited by GW5074. However, GW5074 did not have any significant effect on PE/PDBu-induced MEK/ERK activation. The results indicate that ...Continue Reading

References

Jan 28, 1993·Nature·M J Berridge
Jan 15, 1999·Acta Physiologica Scandinavica·D J Hartshorne
May 5, 1999·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·D S SamuelJ R Florini
May 10, 2000·The Journal of Biological Chemistry·D AbrahamM Baccarini
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P JanoschW Kolch
Nov 24, 2001·The Journal of Biological Chemistry·Constantinos G BroustasUsha Kasid
Apr 10, 2002·The Journal of Biological Chemistry·Yoshihiro IijimaDhandapani Kuppuswamy
Jul 11, 2002·Cell Death and Differentiation·Manuela Baccarini
Sep 12, 2002·European Journal of Pharmacology·Eva V VargaHenry I Yamamura
Jul 9, 2004·The EMBO Journal·Marie C HarrisinghAlison C Lloyd
Nov 30, 2004·Cellular Signalling·Joan C KrepinskyAlistair J Ingram
Dec 14, 2004·Life Sciences·Ming-Ho Tsai, Meei Jyh Jiang
Jan 29, 2005·American Journal of Physiology. Cell Physiology·Roman Ginnan, Harold A Singer
Feb 24, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Katsuya HiranoHideo Kanaide
Jan 27, 2006·American Journal of Physiology. Cell Physiology·Roman GinnanDavid Jourd'heuil
Feb 3, 2006·The Journal of Pharmacology and Experimental Therapeutics·Klaudia BudzynChristopher G Sobey
Feb 28, 2006·Cellular and Molecular Life Sciences : CMLS·E MaioliV Fortino
May 3, 2006·European Journal of Pharmacology·Guogang LuoCang-Bao Xu
Jun 6, 2006·European Journal of Pharmacology·Xu YueWilliam R Roeske
Jul 28, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eikichi IharaJustin A MacDonald
Nov 21, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Radko KomersSharon Anderson
Feb 28, 2008·Physiological Genomics·Liliana Moreno-VinascoJoe G N Garcia

❮ Previous
Next ❯

Citations

May 9, 2014·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Chonglong WuZhice Xu
Apr 1, 2011·Early Human Development·K SathishkumarChandrasekhar Yallampalli
May 4, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Daisuke MaejimaToshio Ohhashi
Dec 25, 2015·Journal of Neurogastroenterology and Motility·Brian A Perrino
Feb 13, 2016·American Journal of Physiology. Cell Physiology·Cindy SutherlandMichael P Walsh
Dec 6, 2019·Journal of Chemical Information and Modeling·Jocelyn SolorzaJans Alzate-Morales

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.