PMID: 7539043Jun 1, 1995Paper

Raf-1 protein is required for growth factor-induced proliferation of hematopoietic cells

The Journal of Experimental Medicine
K W MuszynskiJ R Keller

Abstract

Raf-1 is a 74-kD serine/threonine kinase located in the cell cytoplasm that is activated by phosphorylation in cells stimulated with a variety of mitogens and growth factors, including hematopoietic growth factors. Using c-raf antisense oligonucleotides to block Raf-1 expression, we have established that Raf-1 is required for hematopoietic growth factor-induced proliferation of murine cell lines stimulated by growth factors whose receptors are members of several different structural classes: (a) the hematopoietin receptor family, including interleukin (IL)-2, IL-3, IL-4, granulocyte colony-stimulating factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), and erythropoietin; (b) the tyrosine kinase receptor class, including Steel factor and CSF-1; and (c) IL-6, leukemia inhibitory factor, and oncostatin M, whose receptors include the gp130 receptor subunit. Although results of previous experiments had suggested that IL-4 does not phosphorylate or activate the Raf-1 kinase, c-raf antisense oligonucleotides inhibited IL-4-induced proliferation of both myeloid and T cell lines, and IL-4 activated Raf-1 kinase activity in an IL-4-dependent myeloid cell line. In colony assays, c-raf antisense oligonucleotides completely ...Continue Reading

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Citations

Jun 1, 2001·Advances in Enzyme Regulation·J A McCubreyS C Pohnert
Jun 1, 2000·Advances in Enzyme Regulation·J A McCubreyW L Blalock
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin
Apr 13, 2004·Oncogene·Mandayam O NandanVincent W Yang
May 2, 2006·Molecular and Cellular Biology·Nader OmidvarRichard L Darley
Dec 2, 1999·Experimental Cell Research·C Hagemann, U R Rapp
Dec 20, 2003·The Journal of Biological Chemistry·Weicheng LiuJianxin Gu
Mar 6, 1999·The Journal of Biological Chemistry·S L SutorL M Karnitz
May 21, 1999·Biochemical and Biophysical Research Communications·S V SubramaniamS E Adunyah

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