Raf and fibroblast growth factor phosphorylate Elk1 and activate the serum response element of the immediate early gene pip92 by mitogen-activated protein kinase-independent as well as -dependent signaling pathways.

Molecular and Cellular Biology
K C ChungM R Rosner

Abstract

Previous studies have shown that a mitogen activated protein (MAP) kinase (MEK)-independent signaling pathway is required by activated Raf or fibroblast-derived growth factor (FGF) for the differentiation of rat hippocampal neuronal H19-7 cells. We now demonstrate that both Raf and FGF similarly induce prolonged transcription and translation of the immediate early gene pip92 in the absence of activation of the MAP kinases (MAPKs) ERK1 and ERK2. To determine the mechanism by which this occurs and to identify novel Raf-activated signaling pathways, we investigated the induction of the pip92 promoter by both FGF and an estradiol-activated Raf-1-estrogen receptor fusion protein (deltaRaf-1:ER) in H19-7 cells. Deletion analysis of the pip92 promoter indicated that activation by the MAPK-independent pathway occurs primarily within the region containing a serum response element (SRE). Further analysis of the SRE by using a heterologous thymidine kinase promoter showed that both an Ets and CArG-like site are required. Elk1, which binds to the Ets site, was phosphorylated both in vitro and in vivo by the MAPK-independent pathway, and phosphorylation of an Elk1-GAL4 fusion protein by this pathway was sufficient for transactivation. Final...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E M EvesB H Wainer
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C GallegoG L Johnson
Jul 1, 1992·Trends in Biochemical Sciences·K MacleodD Stehelin
May 1, 1992·Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research·A SethT S Papas
Dec 1, 1990·Molecular and Cellular Biology·C H CharlesL F Lau
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C ColecloughI Lefkovits
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Apr 1, 1993·Trends in Biochemical Sciences·E Nishida, Y Gotoh
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K W WoodS Halegoua
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C H CharlesN K Tonks
Apr 1, 1996·Molecular and Cellular Biology·W L KuoM R Rosner
Jun 28, 1996·The Journal of Biological Chemistry·P LenormandJ Pouysségur
Nov 15, 1996·Cell·H G WangJ C Reed

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Citations

Apr 29, 2008·Brain Research Reviews·Megumi Sugahara KobayashiYasuo Takahashi
Apr 19, 2016·Biochemical and Biophysical Research Communications·J Lee FranklinJoseph L Messina
Apr 8, 1999·Molecular and Cellular Neurosciences·A SurguchovW Baehr
May 3, 2016·Journal of Chemical Neuroanatomy·Shogo MoriyaIshwar S Parhar
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Andrew I SuPeter G Schultz
Jan 10, 2008·Journal of Neuroscience Research·Doo-Yi OhJoong-Soo Han
Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Arturo Hernandez
Dec 13, 2006·Journal of Neurochemistry·Jung Bum ParkKwang Chul Chung
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·A D Sharrocks
Feb 15, 2001·Oncogene·J S Yordy, R C Muise-Helmericks
Jul 17, 2007·The Journal of Biological Chemistry·Lesa A BegleyJill A Macoska
Dec 26, 2001·The Journal of Biological Chemistry·Timothy E GrahamRichard I Dorin
Oct 5, 2002·The Journal of Biological Chemistry·Adam B KeetonJoseph L Messina
May 20, 2017·Hormone Molecular Biology and Clinical Investigation·Junichiro SonodaAmos Baruch
Sep 24, 1999·DNA and Cell Biology·W J LowtherP M Pitha
Nov 27, 2004·Journal of Virology·Sun-Mi KimJae-Hwan Nam
Oct 19, 1999·Gene·M A MohideenM Nilsen-Hamilton
May 22, 2003·The International Journal of Biochemistry & Cell Biology·Peter E Shaw, Janice Saxton
Dec 25, 2003·Gene·Gilles BuchwalterBohdan Wasylyk

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