RAF1 is increased in labouring myometrium and modulates inflammation-induced pro-labour mediators

Reproduction : the Official Journal of the Society for the Study of Fertility
Martha Lappas

Abstract

Inflammation plays a central role in the terminal process of human labour and delivery, including myometrial contractions. RAF1 proto-oncogene serine/threonine-protein kinase (RAF1) can activate ERK (official gene symbol MAPK1) and/or nuclear factor-kappa B (NF-κB) to regulate genes involved in inflammation. There are, however, no studies on the role of RAF1 in the processes of human labour and delivery. Thus, the aims of this study were to determine the effect of i) human labour and pro-inflammatory cytokines interleukin 1 beta (IL1B) and tumour necrosis factor (TNF) alpha on RAF1 protein expression in myometrium and ii) siRNA knockdown of RAF1 on pro-inflammatory and pro-labour mediators in human myometrial primary cells. Term labour was associated with an increase in RAF1 protein expression. Furthermore, RAF1 protein expression was increased in myometrial cells treated with IL1B and TNF, two likely factors contributing to preterm birth. Knockdown of RAF1 by siRNA in primary myometrial cells significantly decreased IL1B- and TNF-induced IL1A, IL1B, IL6, (C-X-C motif) ligand 8 (CXCL8)and chemokine (C-C motif) ligand 2 (CCL2) mRNA abundance and IL6, IL8 and CCL2; prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA levels and pro...Continue Reading

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Mar 15, 2014·Reproductive Health·Hannah BlencoweUNKNOWN Born Too Soon Preterm Birth Action Group
Feb 18, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Martha Lappas

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Citations

May 7, 2020·Metallomics : Integrated Biometal Science·Dineli Matheesha KalansuriyaMartha Lappas
Nov 8, 2018·Mediators of Inflammation·Stella LiongMartha Lappas
Mar 11, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Martha Lappas

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