RAIN-Droplet: a novel 3D in vitro angiogenesis model.

Laboratory Investigation; a Journal of Technical Methods and Pathology
Benjamin D ZeitlinJacques E Nör

Abstract

Angiogenesis is fundamentally required for the initialization, development and metastatic spread of cancer. A rapidly expanding number of new experimental, chemical modulators of endothelial cell function have been described for the therapeutic inhibition of angiogenesis in cancer. Despite this expansion, there has been very limited parallel growth of in vitro angiogenesis models or experimental tools. Here we present the Responsive Angiogenic Implanted Network (RAIN)-Droplet model and novel angiogenesis assay using an endothelial cell culture model of microvascular endothelial cells encapsulated in a spontaneously self-assembling, toroidal hydrogel droplet uniquely yielding discrete, pre-formed, angiogenic networks that may be embedded in 3D matrices. On embedding, radial growth of capillary-like sprouts and cell invasion was observed. The sprouts formed not only as outgrowths from endothelial cells on the surface of the droplets, but also, uniquely, from the pre-formed network structures within the droplet. We demonstrate proof of principle for the utility of the model showing significant inhibition of sprout formation (P<0.001) in the presence of bevacizumab, an anti-angiogenic antibody. Using the RAIN-Droplet assay, we also...Continue Reading

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Citations

Jan 8, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yoanne MousseauFranck G Sturtz
Jun 21, 2014·Future Oncology·Parul Benien, Archana Swami
Oct 23, 2013·Expert Opinion on Drug Discovery·Lauren KimlinVictoria Virador
Nov 21, 2014·Journal of Anatomy·Eleanor Knight, Stefan Przyborski
Sep 18, 2018·Advanced Biosystems·Kristie M TevisMark W Grinstaff
Aug 30, 2018·Stem Cells International·Kevin DzoboKeolebogile Shirley Caroline M Motaung

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