PMID: 11607332Oct 15, 1992Paper

Rainbow trout chimeras produced by injection of blastomeres into recipient blastulae

Proceedings of the National Academy of Sciences of the United States of America
E E Nilsson, J G Cloud

Abstract

In mammals, the ability to successfully introduce isolated cells into a recipient embryo and to document their development has been an important experimental advance in determining the developmental potential of cells. These techniques are also useful in reestablishing the genome of embryonic cells into a germ line. The objective of the present study was to determine whether blastomeres isolated from rainbow trout (Oncorhynchus mykiss) will incorporate and continue to develop when injected into recipient embryos. In the first experiment, donor cells, previously labeled by injecting fluorescein isothiocyanate-dextran into the zygote, were isolated from blastulae; approximately 1000 of these cells were microinjected into each unlabeled recipient embryo of the same developmental stage. Following subsequent development through gastrulation, microscopic examination revealed that 19 of 114 injected embryos (17%) contained fluorescent cells. These labeled cells were present at numerous sites within embryos, and the pattern of distribution of these cells varied among embryos. In experiment two, blastomeres from normal diploid embryos were injected into triploid blastulae. The injected embryos were incubated until hatching and then sacr...Continue Reading

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Citations

Jul 27, 2001·Molecular Reproduction and Development·Y TakeuchiT Takeuchi
Nov 26, 2002·Cryobiology·Satoshi KusudaNobuhisa Koide
Dec 10, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gary H ThorgaardYniv Palti
Feb 16, 2006·Reproduction, Fertility, and Development·J W StoddardJ J Nagler
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y HongM Schartl
Oct 1, 2009·Reproduction in Domestic Animals = Zuchthygiene·M Francisco-SimãoF García-Ximénez
May 5, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·N C WendlingR L Ingermann
Dec 3, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Igor Babiak, Konrad Dabrowski

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