RALF1-FERONIA complex affects splicing dynamics to modulate stress responses and growth in plants.

Science Advances
Long WangFeng Yu

Abstract

The environmentally responsive signaling pathways that link global transcriptomic changes through alternative splicing (AS) to plant fitness remain unclear. Here, we found that the interaction of the extracellular rapid alkalinization FACTOR 1 (RALF1) peptide with its receptor FERONIA (FER) triggered a rapid and massive RNA AS response by interacting with and phosphorylating glycine-rich RNA binding protein7 (GRP7) to elevate GRP7 nuclear accumulation in Arabidopsis thaliana. FER-dependent GRP7 phosphorylation enhanced its mRNA binding ability and its association with the spliceosome component U1-70K to enable splice site selection, modulating dynamic AS. Genetic reversal of a RALF1-FER-dependent splicing target partly rescued mutants deficient in GRP7. AS of GRP7 itself induced nonsense-mediated decay feedback to the RALF1-FER-GRP7 module, fine-tuning stress responses, and cell growth. The RALF1-FER-GRP7 module provides a paradigm for regulatory mechanisms of RNA splicing in response to external stimuli.

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Citations

Aug 26, 2020·Science China. Life Sciences·Long WangFeng Yu
Sep 2, 2020·Biochemical Society Transactions·Paola PunzoGiorgia Batelli
Oct 21, 2020·International Journal of Molecular Sciences·Zhe Wang, Xiaoping Gou
Oct 30, 2020·International Journal of Molecular Sciences·Dongchao JiShiping Tian

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Methods Mentioned

BETA
RNA-seq
Y2H
pull-down
Co-IP
two-hybrid
fluorescence complementation
transgenic
RIP
electrophoretic mobility shift assay
ubiquitination

Software Mentioned

BiFC
htseq
count
Proteome Discoverer
SPSS
DESeq
MEME
MaxQuant
DNAMAN
Trimmomatic

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