Raloxifene increases the capacity of serum to promote prostacyclin release in human endothelial cells: implication of COX-1 and COX-2

Menopause : the Journal of the North American Menopause Society
Pilar J OviedoAntonio Cano

Abstract

Prostacyclin is a potent vasodilator synthesized by two isoforms of cyclooxygenase in endothelium. The aim of this study was to investigate the effect of serum from postmenopausal women treated with raloxifene on prostacyclin production by human umbilical vein endothelial cells and on cyclooxygenases-1 and -2. Serum was collected from 21 women receiving 60 mg/day of raloxifene, at baseline and at 3 and 6 months. Human umbilical vein endothelial cells were exposed to serum for 24 hours, and prostacyclin production was evaluated in supernatants. Selective inhibitors of cyclooxygenases-1 and -2 (SC-560 and NS-398) were used to investigate the relative contribution of each enzyme. Protein expression for each enzyme was determined using Western blot assays. Prostacyclin production was increased by 30% (P < or = 0.001) when serum from women treated for 3 and 6 months was added. SC-560 reversed prostacyclin production but did not change baseline values. NS-398, in turn, reduced prostacyclin production using sera from baseline, 3 and 6 months. Cyclooxygenases-1 and -2 protein expression remained unaltered at each treatment step. Serum from women treated with raloxifene stimulated prostacyclin release from human umbilical vein endotheli...Continue Reading

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Citations

Jun 30, 2007·Clinical and Experimental Pharmacology & Physiology·Fung Ping LeungYu Huang
Apr 25, 2007·Climacteric : the Journal of the International Menopause Society·A CanoJ J Tarín
May 7, 2010·Climacteric : the Journal of the International Menopause Society·P J OviedoC Hermenegildo
Sep 30, 2008·Climacteric : the Journal of the International Menopause Society·D BruceO Ylikorkala
Jan 31, 2017·Biochemical Pharmacology·Agua SobrinoCarlos Hermenegildo
Aug 19, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiara BolegoAndrea Cignarella
Jan 23, 2021·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Darya DudenkoAntonio Cano
Jun 6, 2007·Menopause : the Journal of the North American Menopause Society·Rami Abu-FanneHaim D Danenberg

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