Raman analysis of prion protein in blood cell membranes from naturally affected scrapie sheep

Chemistry & Biology
Pedro CarmonaJaime Monreal

Abstract

At present, there are no efficient antemortem diagnostic tests for transmissible spongiform encephalopathys (TSEs), particularly in blood. New strains of prion PrPSc, which causes TSEs, are currently appearing, and researchers remain concerned that if prion variants continue to emerge, some of them may escape detection by existing immunoassay tests. Because a common structural feature of PrPSc strains is their high content of beta sheets, Raman spectroscopy has proven to be a suitable technique to analyze a key membranous fraction of blood containing PrPSc. In this fraction, a significant increase in beta sheets has been correlated with the worsening of this TSE in naturally scrapie-infected animals in comparison with healthy controls. Since sensitivity and specificity were found to be 100% for each, this test may lead to a new and alternative diagnosis for prion diseases.

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Citations

Apr 12, 2017·Applied Spectroscopy·Chad G AtkinsRobin F B Turner
Feb 21, 2008·Veterinary Research·Jacques GrassiNathalie Morel
May 21, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Benjamin D RoachBridgette J Duncombe
Jul 11, 2006·Expert Review of Molecular Diagnostics·Marty T LehtoNeil R Cashman
Mar 16, 2007·Journal of Neuropathology and Experimental Neurology·Ana Patricia FernándezAlfredo Martínez
Nov 22, 2005·The Journal of General Virology·Pedro CarmonaJaime Monreal
May 12, 2005·The Biochemical Journal·Alana M ThackrayRaymond Bujdoso
Dec 12, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fangfang ChenXiaoyi Lv
Jan 9, 2018·ACS Chemical Neuroscience·George DevittSumeet Mahajan

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