PMID: 6162474Dec 23, 1980Paper

Raman and 19F(1H) nuclear Overhauser evidence for a rigid solution conformation of Escherichia coli 5-fluorouracil 5S ribonucleic acid

Biochemistry
A G Marshall, J L Smith

Abstract

Escherichia coli cells grown on a medium containing 5-fluorouracil (FU) produce 5S RNA whose uracil residues are approximately 80% replaced by FU. The Raman spectra of native and FU-5S RNA are very similar, confirming similar solution conformations for the two species and a highly base-stacked structure in solution. The 254-MHz 19F NMR spectrum of FU-5S RNA shows that the 20-odd FU residues reside in at least ten distinct chemical environments, suggesting a highly ordered structure. Comparison of theoretical and experimental 19F(1H) nuclear Overhauser enhancements demonstrates definitively that virtually all the labeled uracils are bound to a rigid macromolecular frame, with a rotational correlation time of about 19 ns or longer. Since these uracils are widely distributed throughout the nucleotide primary sequence, it may be concluded that the entire FU-5S RNA solution structure is relatively rigid, in agreement with the most recently proposed "cloverleaf" secondary structural model for native prokaryotic 5S RNA.

References

Jan 19, 1977·Journal of the American Chemical Society·A G Marshall, J L Smith
Oct 15, 1978·Journal of Molecular Biology·G A Luoma, A G Marshall
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G A Luoma, A G Marshall
Jan 1, 1979·Nucleic Acids Research·V A Erdmann
Oct 15, 1975·FEBS Letters·N DelihasV A Erdmann
Aug 7, 1975·Nature·G E Fox, C R Woese
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B D SykesM J Schlesinger
May 31, 1972·Journal of the American Chemical Society·P BalaramJ Dadok
Aug 13, 1968·Biochemical and Biophysical Research Communications·M Litt
Sep 30, 1980·Biochemical and Biophysical Research Communications·P D BurnsA G Marshall
Mar 19, 1965·Science·R W HOLLEYA ZAMIR

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Citations

May 24, 1985·Biochimica Et Biophysica Acta·I I KaiserD A Frendewey
Mar 4, 2000·Journal of Biomolecular Structure & Dynamics·L C Sowers
Nov 7, 1998·The Journal of Biological Chemistry·E Dahan-GrobgeldH Degani

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