Raman Imaging of Individual Membrane Lipids and Deoxynucleoside Triphosphates in Living Neuronal Cells during Neurite Outgrowth

ACS Chemical Neuroscience
Giuseppe PezzottiOsam Mazda

Abstract

Recent developments in Raman imaging at the microscopic scale were exploited here with the specific purpose of locating spectral fingerprints of individual membrane lipids and deoxynucleoside triphosphates during neuronal cell networking and separation. After carefully screening the Raman spectra of isolated lipid components, we located an in situ mapped specific Raman fingerprints from individual phospholipids at the micrometric level in comparison with the total lipid distribution within single living cells. We concurrently examined silent zones of lipid emissions and exploited those peculiar spectral ranges for mapping both abundance and localization of individual DNA nucleoside triphosphates. This work represents a first step toward label-free/molecular-selective Raman patterning with high spectral resolution of the relevant chemical species involved with the functionality of neuronal cells.

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Citations

Oct 20, 2018·The Analyst·Lei YinJingkai Gu
Jan 15, 2021·Chemistry, an Asian Journal·Adukkadan N RamyaKaustabh K Maiti
Jun 30, 2019·ACS Applied Materials & Interfaces·Giuseppe Pezzotti
Jul 1, 2020·ACS Chemical Neuroscience·Giuseppe PezzottiOsam Mazda

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