Raman microspectroscopy and Raman imaging reveal biomarkers specific for thoracic aortic aneurysms.

Cell Reports Medicine
Kaori SugiyamaHiromi Yanagisawa

Abstract

Aortic rupture and dissection are life-threatening complications of ascending thoracic aortic aneurysms (aTAAs), and risk assessment has been largely based on the monitoring of lumen size enlargement. Temporal changes in the extracellular matrix (ECM), which has a critical impact on aortic remodeling, are not routinely evaluated, and cardiovascular biomarkers do not exist to predict aTAA formation. Here, Raman microspectroscopy and Raman imaging are used to identify spectral biomarkers specific for aTAAs in mice and humans by multivariate data analysis (MVA). Multivariate curve resolution-alternating least-squares (MCR-ALS) combined with Lasso regression reveals elastic fiber-derived (Ce1) and collagen fiber-derived (Cc6) components that are significantly increased in aTAA lesions of murine and human aortic tissues. In particular, Cc6 detects changes in amino acid residues, including phenylalanine, tyrosine, tryptophan, cysteine, aspartate, and glutamate. Ce1 and Cc6 may serve as diagnostic Raman biomarkers that detect alterations of amino acids derived from aneurysm lesions.

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·F M PopeV A McKusick
Feb 1, 1975·Biopolymers·B G Frushour, J L Koenig
Mar 1, 1995·Analytical Chemistry·C J FrankD C Redd
Jan 5, 1999·European Journal of Biochemistry·L DebelleP Legrand
Mar 4, 2000·Physics in Medicine and Biology·E B HanlonM S Feld
Nov 6, 2003·International Journal of Cancer. Journal International Du Cancer·Zhiwei HuangHaishan Zeng
Dec 13, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·D SajanV S Jayakumar
Jan 24, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Alan Daugherty, Lisa A Cassis
Mar 3, 2004·Faraday Discussions·Nicholas StoneHugh Barr
Oct 20, 2004·IEEE Transactions on Bio-medical Engineering·Sigurdur SigurdssonHans Christian Wulf
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey A SpencerHiromi Yanagisawa
Oct 18, 2005·Microscopy Research and Technique·Wen-Ting ChengShan-Yang Lin
Jul 20, 2006·Journal of the Royal Society, Interface·I NotingherL L Hench
Dec 25, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Evren U AzelogluKevin D Costa
Apr 12, 2008·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Nusrat ChoudhuryRichard L Leask
Feb 13, 2009·Connective Tissue Research·Richard EllisC Peter Winlove
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masahito HoriguchiTomoyuki Nakamura
Feb 4, 2010·Circulation·P E Norman, J T Powell
May 20, 2011·The Journal of Thoracic and Cardiovascular Surgery·Santi TrimarchiKim A Eagle
Jul 22, 2011·Tissue Engineering. Part C, Methods·Marieke PudlasKatja Schenke-Layland
Mar 26, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Eser AdiguzelMichelle P Bendeck
Mar 13, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Christina L Papke, Hiromi Yanagisawa
Jan 27, 2015·Nature Photonics·Charles H CampMarcus T Cicerone
Feb 13, 2015·Science Translational Medicine·Michael JermynFrederic Leblond
Mar 10, 2016·Chemical Society Reviews·Oliver StevensNick Stone
Apr 14, 2016·The Analyst·Rachael SmithLorna Ashton
Jun 15, 2016·Circulation·Eric M IsselbacherMark E Lindsay
Nov 24, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Dianna M MilewiczJames T Stull
Apr 21, 2017·Nature·Lu WeiWei Min
May 12, 2017·Journal of the Royal Society, Interface·C BelliniJ D Humphrey

❮ Previous
Next ❯

Citations

Sep 23, 2021·Analytical Methods : Advancing Methods and Applications·Bukem TanorenMurat Ugurlucan

❮ Previous
Next ❯

Software Mentioned

CAMO
GraphPad
Prism
in
ALS
Unscrambler X10
True Component Analysis ( TCA ) FIVE
MCR
TCA
house

Related Concepts

Related Feeds

Aortic Aneurysm

An aortic aneurysm is the weakening and bulging of the blood vessel wall in the aorta. This causes dilatation of the aorta, which is usually asymptomatic but carries the risk of rupture and hemorrhage. Find the latest research on aortic aneurysms here.

Cardiac Aneurysm

Aneurysm refers to a bulge of the wall or lining of a vessel commonly occurring in the blood vessels at the base of the septum or within the aorta. In the heart, it usually arises from a patch of weakened tissue in a ventricular wall, which swells into a bubble filled with blood. Discover the latest research on cardiac aneurysm here.

Aneurysm

Aneurysms are outward distensions or bulges that occurs in a weakened wall of blood vessels. Discover the latest research on aneurysms here.