Raman spectral cytopathology for cancer diagnostic applications.

Nature Protocols
Damien TraynorFiona Mary Lyng

Abstract

Raman spectroscopy can provide a rapid, label-free, nondestructive measurement of the chemical fingerprint of a sample and has shown potential for cancer screening and diagnosis. Here we report a protocol for Raman microspectroscopic analysis of different exfoliative cytology samples (cervical, oral and lung), covering sample preparation, spectral acquisition, preprocessing and data analysis. The protocol takes 2 h 20 min for sample preparation, measurement and data preprocessing and up to 8 h for a complete analysis. A key feature of the protocol is that it uses the same sample preparation procedure as commonly used in diagnostic cytology laboratories (i.e., liquid-based cytology on glass slides), ensuring compatibility with clinical workflows. Our protocol also covers methods to correct for the spectral contribution of glass and sample pretreatment methods to remove contaminants (such as blood and mucus) that can obscure spectral features in the exfoliated cells and lead to variability. The protocol establishes a standardized clinical routine allowing the collection of highly reproducible data for Raman spectral cytopathology for cancer diagnostic applications for cervical and lung cancer and for monitoring suspicious lesions...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P T WongB Rigas
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M A Cohenford, B Rigas
May 16, 2002·Biopolymers·Max DiemAnthony Pacifico
Dec 5, 2006·Biochemical and Biophysical Research Communications·Michael J WalshFrancis L Martin
Feb 10, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kostas PapamarkakisMax Diem
Mar 13, 2010·Applied Spectroscopy·Lori E KamemotoTayro Acosta
Apr 3, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jemma G KellyFrancis L Martin
Apr 7, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jennifer M SchubertMax Diem
Jun 29, 2012·Translational Oncology·Elizabeth VargisAnita Mahadevan-Jansen
Nov 16, 2012·Analytical and Bioanalytical Chemistry·Katia WehbeGianfelice Cinque
Jan 23, 2015·The Analyst·Hugh J ByrneGanesh D Sockalingum
Mar 11, 2016·Nature Protocols·Holly J ButlerFrancis L Martin
Apr 2, 2016·Faraday Discussions·I R RamosF M Lyng
May 16, 2017·Journal of Gastroenterology·Oliver OldHugh Barr
May 26, 2017·Analytical Chemistry·Hesham K YosefKlaus Gerwert
Nov 1, 2017·Journal of Biomedical Optics·Padraig KearneyCara M Martin
Nov 16, 2017·Journal of Biomedical Optics·Aditi SahuC Murali Krishna
Dec 8, 2017·Journal of Biophotonics·Damien TraynorFiona M Lyng
Feb 6, 2019·Journal of Biophotonics·Aditi SahuC Murali Krishna
Jun 20, 2020·Nature Protocols·Camilo L M MoraisFrancis L Martin
Jan 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hugh J ByrneFiona M Lyng

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Software Mentioned

EMSC
MATLAB PLS Toolbox (
CytoLyt
PLS Toolbox
Python
MATLAB
ThinPrep
NNLS
CytoSpec
Eigenvector )

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