PMID: 7037052Jan 5, 1982Paper

Raman spectral evidence for a mu-oxo bridge in the binuclear iron center of ribonucleotide reductase

Biochemistry
B M SjöbergJ Sanders-Loehr

Abstract

The Raman spectrum of the B2 subunit of Escherichia coli ribonucleotide reductase shows a peak at 496 cm-1 that appears to be in resonance with the 370-nm electronic transition of the binuclear iron center in both the native and radical-free forms of the protein. Exposure of the protein to H218O causes the peak to shift to 481 cm-1, indicating that the vibrational mode is due to an Fe-O moiety in which the oxygen can exchange with solvent. The rate of oxygen exchange (kobsd = 8.3 x 10-4 s-1) is consistent with a mu-oxo-bridged structure. Protonation of the oxygen is unlikely since the Fe-O vibration fails to shift to lower frequency in D2O. Instead, there is a gradual increase in the vibrational frequency with time to a maximum value of 502 cm-1 after 3 h in 70% with time to a maximum value of 602 cm-1 after 3 h in 70% D2O. Apparently, the deuteration of successive protein functional groups causes a slight alteration in the structure of the binuclear iron center. The resonance Raman characteristics of the Fe-O-Fe group in protein B2 are similar to those previously reported for the mu-oxo-bridged binuclear iron center in hemerythrin. A further similarity between the two proteins is the high degree of alpha-helical content. Circu...Continue Reading

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Citations

Feb 9, 2005·Biopolymers·Jennifer R CalhounWilliam F DeGrado
Jan 1, 1983·Pharmacology & Therapeutics·H P Hogenkamp
Feb 14, 2002·Progress in Biophysics and Molecular Biology·H EklundP Nordlund
Apr 22, 2011·Journal of the American Chemical Society·Van V VuLawrence Que
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T BarlowB M Sjöberg
Oct 24, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gary W Brudvig
Dec 1, 1985·Environmental Health Perspectives·A GräslundB M Sjöberg
Jan 22, 2015·Nature·Rahul BanerjeeDenis A Proshlyakov
Jun 19, 1984·Biochemistry·S S Sibbett, J K Hurst
Jun 16, 1987·Biochemistry·P Reichard
May 10, 1991·Biochemical and Biophysical Research Communications·M P HendrichL Que
Mar 20, 2018·Natural Product Reports·Anna J KomorJohn D Lipscomb
Aug 24, 2004·The Journal of Biological Chemistry·Kari R StrandK Kristoffer Andersson
May 11, 2017·Essays in Biochemistry·Steven Y Reece, Mohammad R Seyedsayamdost
Feb 17, 2006·The Journal of Biological Chemistry·Seyed Isaac HashemyArne Holmgren
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Dec 15, 2000·Biochimica Et Biophysica Acta·W Hillier, T Wydrzynski
Jan 1, 1985·Pharmacology & Therapeutics·G W Ashley, J Stubbe
Jul 1, 1986·Journal of Bacteriology·L A ActisJ Sanders-Loehr
Nov 7, 1996·Chemical Reviews·Richard H. HolmEdward I. Solomon
Aug 5, 1983·Science·P Reichard, A Ehrenberg

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