PMID: 3768324Aug 26, 1986Paper

Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides

Biochemistry
K T YueR Callender

Abstract

We have measured the Raman spectra of oxidized nicotinamide adenine dinucleotide, NAD+, and its reduced form, NADH, as well as a series of fragments and analogues of NAD+ and NADH. In addition, we have studied the effects of pH as well as deuteration of the exchangeable protons on the Raman spectra of these molecules. In comparing the positions and intensities of the peaks in the fragment and analogue spectra with those of NADH and NAD+, we find that it is useful to consider these large molecules as consisting of component parts, namely, adenine, two ribose groups, two phosphate groups, and nicotinamide, for the purpose of assigning their spectral features. The Raman bands of NADH and NAD+ are found generally to arise from molecular motions in one or another of these molecular moieties, although some peaks are not quite so easily identified in this way. This type of assignment is the first step in a detailed understanding of the Raman spectra of NAD+ and NADH. This is needed to understand the binding properties of NADH and NAD+ acting as coenzymes with the NAD-linked dehydrogenases as deduced recently by using Raman spectroscopy.

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Citations

Sep 10, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xiaodi YangXianlong Wang
Jul 2, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaodi YangZhengbiao Yang
Sep 9, 2015·Journal of Inorganic Biochemistry·Elena FormosoXabier Lopez
Sep 26, 2012·Colloids and Surfaces. B, Biointerfaces·Li LiRen Fang Shen
May 7, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Vishakha KarnawatMrinalini Puranik
Dec 10, 2013·The Journal of Physical Chemistry. B·Niall SimpsonNeil T Hunt
Sep 22, 2016·Lasers in Medical Science·Guilherme Akio Tamura OzakiJosé Carlos Silva Camargo Filho
Feb 9, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Alexis Damian, Sasha Omanovic
Mar 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Carmen L RecksiedlerMichael S Freund

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