Ramifications of kinetic partitioning on usher-mediated pilus biogenesis

The EMBO Journal
E T SaulinoS J Hultgren

Abstract

The biogenesis of diverse adhesive structures in a variety of Gram-negative bacterial species is dependent on the chaperone/usher pathway. Very little is known about how the usher protein translocates protein subunits across the outer membrane or how assembly of these adhesive structures occurs. We have discovered several mechanisms by which the usher protein acts to regulate the ordered assembly of type 1 pili, specifically through critical interactions of the chaperone-adhesin complex with the usher. A study of association and dissociation events of chaperone-subunit complexes with the usher in real time using surface plasmon resonance revealed that the chaperone-adhesin complex has the tightest and fastest association with the usher. This suggests that kinetic partitioning of chaperone-adhesin complexes to the usher is a defining factor in tip localization of the adhesin in the pilus. Furthermore, we identified and purified a chaperone-adhesin-usher assembly intermediate that was formed in vivo. Trypsin digestion assays showed that the usher in this complex was in an altered conformation, which was maintained during pilus assembly. The data support a model in which binding of the chaperone-adhesin complex to the usher stabil...Continue Reading

References

Jan 1, 1990·Molecular & General Genetics : MGG·P Klemm, G Christiansen
Sep 5, 1973·Journal of Molecular Biology·Y MitsuiR Langridge
Jun 1, 1995·Trends in Microbiology·M Russel
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·C H JonesS J Hultgren
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J A RobertsS Normark
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K W DodsonS J Hultgren
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C H JonesS J Hultgren
Jun 1, 1995·Molecular Microbiology·Q A ValentB Oudega
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·I ConnellC Svanborg
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·E BullittS J Hultgren
Dec 1, 1996·Trends in Microbiology·G P Salmond
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G ThanassiS J Hultgren

❮ Previous
Next ❯

Citations

Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Sep 25, 2002·Journal of Molecular Biology·Michael VetschRudi Glockshuber
Jan 21, 2000·FEMS Microbiology Reviews·L A Fernández, J Berenguer
Jun 30, 2000·Current Opinion in Cell Biology·D G Thanassi, S J Hultgren
Oct 24, 2000·Current Opinion in Structural Biology·F G SauerS J Hultgren
Dec 5, 2000·Current Opinion in Chemical Biology·S D KnightD Choudhury
Feb 19, 2000·Current Opinion in Microbiology·F G SauerS J Hultgren
Oct 13, 2009·Nature Reviews. Microbiology·Gabriel Waksman, Scott J Hultgren
Apr 16, 2002·Molecular Microbiology·Anne-Laure PageClaude Parsot
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rebecca J RoseSheena E Radford
Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yihua HuangJohann Deisenhofer
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ender VolkanScott J Hultgren
Sep 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Drew J SchwartzScott J Hultgren
Oct 24, 2009·The Journal of Biological Chemistry·Owen S MapingireAnne H Delcour
Mar 1, 2012·Molecular & Cellular Proteomics : MCP·Bethny MorrisseySheena E Radford
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Andreas Busch, Gabriel Waksman
Mar 29, 2008·Science·Mireille NishiyamaRudi Glockshuber
May 15, 2007·Journal of Bacteriology·Yvonne M LeeScott J Hultgren
Feb 2, 2010·Journal of Bacteriology·Bradley FordGabriel Waksman
Sep 25, 2012·Journal of Bacteriology·Bradley FordScott Hultgren
Oct 26, 2002·Journal of Bacteriology·David G ThanassiScott J Hultgren
Apr 18, 2003·Journal of Bacteriology·Michelle M BarnhartScott J Hultgren
Jun 19, 2004·Journal of Bacteriology·Yvonne M LeeScott J Hultgren
Jun 22, 2005·Journal of Bacteriology·Maria KostakiotiChristos Stathopoulos
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Sean-Paul Nuccio, Andreas J Bäumler
Sep 24, 2004·Journal of Molecular Microbiology and Biotechnology·F VerdonckB M Goddeeris
May 15, 2013·PloS One·Diana C F MonteiroEmanuele Paci
May 22, 2007·PLoS Pathogens·Denis VergerGabriel Waksman
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ender VolkanScott J Hultgren
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jerome S PinknerFredrik Almqvist
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·E T SaulinoS J Hultgren
Feb 27, 1999·Molecular Microbiology·D L HungS J Hultgren
May 7, 2014·Biochimica Et Biophysica Acta·James LillingtonGabriel Waksman
Jul 10, 2012·Nature Chemical Biology·Maria D CrespoRudi Glockshuber
Apr 10, 2010·Trends in Microbiology·Kimberly A KlineScott J Hultgren

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.