RAMPs and CRLR expressions in osteoblastic cells after dexamethasone treatment

Biochemical and Biophysical Research Communications
Benjamin UzanMichèle Cressent

Abstract

Adrenomedullin (ADM) is a potent stimulator of osteoblastic activity and promotes bone growth in vivo. ADM receptors are formed by heterodimerization of the CRLR and a RAMP2 or RAMP3 molecule. Since glucocorticoid responsive elements were recently identified in the human CRLR promoter and that glucocorticoids exert a major action in bones, we investigated the acute effect of dexamethasone (Dex) treatment on ADM receptor components in osteoblastic cell types: the MC3T3-E1 cells and calvaria-derived osteoblastic cells. Changes in expression of CRLR and RAMPs molecules were evaluated at mRNA levels using RT-PCR and at protein levels by Western blot analysis. We found that Dex increased expression of RAMP1 and RAMP2 mRNA in a time-dependent but dose-independent manner, while RAMP3 was unchanged. In contrast, Dex decreased the CRLR mRNA expression and these changes were reflected at protein levels. We suggest that Dex, in osteoblastic cells, altered ADM receptor by inhibition of CRLR expression and consequently could impair the ADM anabolic effect on bone. Our findings could explain in part, the detrimental side effects observed at bone level during glucocorticoid therapy.

References

Apr 9, 1999·Molecular and Cellular Endocrinology·Y HattoriK Kasai
Apr 25, 2000·Biochemical and Biophysical Research Communications·S FrayonJ M Garel
Apr 25, 2000·Biochemical and Biophysical Research Communications·Y OnoK Kangawa
Mar 21, 2001·Cellular Signalling·P M SextonN Tilakaratne
Jun 8, 2002·Biochemical and Biophysical Research Communications·Carine CueilleJean-Michel Garel
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Leonid L NikitenkoMargaret C P Rees
Nov 11, 2003·Trends in Pharmacological Sciences·Maria MorfisPatrick M Sexton
Jan 9, 2004·International Journal of Cancer. Journal International Du Cancer·Samantha Fernandez-SauzeFrançoise Boudouresque

❮ Previous
Next ❯

Citations

Nov 1, 2011·Cellular and Molecular Neurobiology·Tessei NagayamaHiroyuki Ichikawa
Oct 8, 2013·The International Journal of Biochemistry & Cell Biology·Elisabetta Liverani, Carolyn Paul
May 13, 2014·General and Comparative Endocrinology·Rute MartinsDeborah M Power
Jan 17, 2015·Molecular and Cellular Endocrinology·Silvia R TerraVanessa Schein
Jun 20, 2012·Advanced Drug Delivery Reviews·Kazuhiro AokiKeiichi Ohya
Oct 18, 2007·Journal of Cellular Physiology·Benjamin UzanMichèle Cressent
Aug 10, 2011·Journal of Cellular Biochemistry·Sylvie Lausson, Michèle Cressent
Oct 3, 2006·Trends in Biochemical Sciences·Narayanan Parameswaran, William S Spielman
Feb 7, 2006·Regulatory Peptides·Iván Márquez-RodasGloria Balfagón
Aug 23, 2005·Pharmacology & Therapeutics·Debbie L HayPatrick M Sexton
Mar 22, 2006·Expert Opinion on Therapeutic Targets·Mario A GarcíaAlfredo Martínez
Jun 27, 2012·International Immunopharmacology·Elisabetta LiveraniCarolyn Paul
Apr 30, 2017·International Journal of Molecular Sciences·Susanne Grässel, Dominique Muschter
Dec 13, 2018·Physiological Reviews·Dorit NaotJillian Cornish
Sep 20, 2007·American Journal of Physiology. Endocrinology and Metabolism·Alina SilaghiMichel Grino
Apr 14, 2017·Connective Tissue Research·Yao LiuEn Luo
Sep 24, 2020·International Journal of Endocrinology·Jingbo XieQingshan Sun
Oct 9, 2020·Current Osteoporosis Reports·Jiankun XuLing Qin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.