Random amplification of polymorphic DNA as a tool for taxonomic studies of triatomine bugs (Hemiptera: Reduviidae)

Journal of Medical Entomology
A L GarciaM A Miles

Abstract

Eleven of 27 decameric primers were found to be suitable for random amplification of polymorphic DNA (RAPD) from triatomine bugs on the basis that they produced discrete profiles and distinguished among Panstrongylus megistus (Burmeister), Rhodnius prolixus Stål, and Triatoma infestans (Klug). The legs, or single leg segments, of individual bugs were used as the source of DNA so that the taxonomic value of the bug was conserved. Within the scope of the specimens studied, RAPD profiles allowed assignment to species even when bugs were kept dry for up to 12 mo. Profiles for individuals within a species were not identical. RAPD profiles, with the specimens tested, distinguished among species of 3 pairs considered to be morphologically similar and closely related, namely, Rhodnius ecuadorensis Lent & León and Rhodnius pictipes Stål; Rhodnius nasutus Stål, and Rhodnius neglectus Lent; Rhodnius prolixus Stål and Rhodnius robustus Larrousse. RAPD data conformed with the perceived affinities among these species. RAPD polymorphisms were seen with T. infestans from 3 different localities, but none of the polymorphisms was confined to 1 source. RAPD provided a molecular basis to reassess taxonomic relationships within the Triatomine subfa...Continue Reading

Citations

Oct 7, 2014·Frontiers in Public Health·Nilsa E Gonzalez-BritezAntonieta Rojas de Arias
May 17, 2011·PLoS Neglected Tropical Diseases·Miriam RolónAntonieta Rojas de Arias
Apr 20, 2002·Medical and Veterinary Entomology·F A MonteiroA M Solé-Cava
Mar 18, 2011·Revista Do Instituto De Medicina Tropical De São Paulo·Jorge FragaAymé Fernández-Calienes
Dec 19, 2000·Cadernos de saúde pública·C J Schofield
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·C J SchofieldJ P Dujardin
Aug 30, 2005·Anais Da Academia Brasileira De Ciências·Fernando Abad-Franch, Fernando A Monteiro
Nov 23, 2005·Revista Do Instituto De Medicina Tropical De São Paulo·Jorge FragaMayda Castex
Mar 28, 2008·Memórias do Instituto Oswaldo Cruz·Rita de Cássia Moreira de SouzaLiléia Diotaiuti
Apr 12, 2007·Memórias do Instituto Oswaldo Cruz·Sergio MelgarCarlota Monroy
Jun 29, 2015·Briefings in Functional Genomics·David Roy Smith

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