PMID: 8972084Dec 1, 1996Paper

Random amplification of polymorphic DNA (RAPD) of Salmonella: strain differentiation and characterization of amplified sequences

The Journal of Applied Bacteriology
A C HiltonC W Penn

Abstract

Random amplification of polymorphic DNA (RAPD) was evaluated for its ability to differentiate Salmonella strains from various sources. Under defined conditions RAPD using a 10-mer primer (1254) produced a series of amplification products able to reproducibly distinguish strains representing 20 different serotypes of Salmonella. Primer 1254 also proved capable of discrimination between some but not all isolates of Salm. ser. Enteritidis and Salm. ser. Typhimurium, phage typing proving to be most discriminatory for the latter serotype. Cloning of fragments into a vector allowed sequencing and database searching for identification of fragments and an indication of criteria for primer template interaction in RAPD. Southern blotting using a digoxigenin-labelled probe allowed identification of related bands between RAPD profiles. These observations demonstrate the potential of rapid molecular typing by RAPD for the genomic typing of Salmonella strains.

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Citations

Oct 1, 2010·Indian Journal of Microbiology·Lopamudra SahooS C Mukherjee
Apr 21, 1999·Research in Microbiology·E MoriR Fani
Jul 9, 2002·Letters in Applied Microbiology·M H R KhoodooY Jaufeerally-Fakim
Nov 13, 2003·FEMS Microbiology Letters·Alexandra L PerryTom S J Elliott
Apr 20, 2004·Revista Da Sociedade Brasileira De Medicina Tropical·Bianca Ramalho QuintaesErnesto Hofer
Sep 27, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M RuizG Royo

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