PMID: 11607517Mar 14, 1995Paper

Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine

Proceedings of the National Academy of Sciences of the United States of America
M E DeveyDavid B Neale

Abstract

We have genetically mapped a gene for resistance to white pine blister rust (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) by using an approach which relies on three factors: (i) the ability to assay for genetic markers in the haploid stage of the host's life cycle, using megagametophyte seed tissue; (ii) a simple and clearly defined pathosystem; and (iii) the use of random amplified polymorphic DNA (RAPD) markers that can be quickly and efficiently evaluated. Resistance to white pine blister rust in sugar pine is known to be controlled by a single dominant gene (R). Maternal segregation of R and dominant RAPD markers were scored simultaneously following collection of megagametophytes for DNA assays and seedling inoculation with C. ribicola. Bulked samples of haploid megagametophyte DNA from resistant and susceptible offspring of segregating full-sib and half-sib families were used to evaluate 800 random decanucleotide primers. Ten loci linked with the gene for resistance to white pine blister rust were identified and segregation data were obtained from five families. Six of the linked markers were within 5 centimorgans of the gene, and one marker was 0.9 centimorgan from R. These and other markers derive...Continue Reading

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Citations

Oct 24, 2008·Phytopathology·R C HamelinK Et-Touil
Oct 24, 2008·Phytopathology·Bohun B Kinloch
Dec 1, 1996·Genome Génome / Conseil National De Recherches Canada·C S EchtR Zahorchak
May 21, 2013·Annual Review of Phytopathology·Andriy KovalchukFred O Asiegbu
Apr 11, 2009·Journal of Electromyography and Kinesiology : Official Journal of the International Society of Electrophysiological Kinesiology·Javier Rodríguez-FalcesJavier Navallas
Dec 7, 2007·Current Opinion in Genetics & Development·David B Neale
Sep 25, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D T JunghansD Grattapaglia
Apr 7, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E HayashiS Kawasaki
Oct 18, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D ZhangB Li
Nov 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A LehnerD B Neale
Oct 30, 2016·Genetics·Kristian A StevensCharles H Langley

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