Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India.

Journal of Veterinary Science
J P SinghP Chaudhuri

Abstract

The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied.

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Citations

Jul 4, 2012·Vaccine·Katarzyna Krysztopa-GrzybowskaAnna Lutyńska
Feb 23, 2010·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·V V RozhnovV G Yudin
Dec 20, 2018·International Journal of Veterinary Science and Medicine·Ahmad FawzyTobias Eisenberg
Sep 6, 2008·Canadian Journal of Microbiology·G Vázquez-MarrufoM S Vázquez-Garcidueñas
Mar 26, 2021·Infection and Drug Resistance·Shaymaa H Abdel-Rhman, Dina E Rizk

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Datasets Mentioned

BETA
IS1081
IS6110

Methods Mentioned

BETA
biotyping
PCR
RAPD

Software Mentioned

RAPDistance
AlfaImager

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