Random Motion of Chromatin Is Influenced by Lamin A Interconnections

Biophysical Journal
Fereydoon TaheriKatalin Tóth

Abstract

Using fluorescence correlation spectroscopy in single-plane illumination microscopy, we investigated the dynamics of chromatin in interphase mouse adult fibroblast cell nuclei under the influence of the intermediate filament protein lamin A. We find that 1) lamin A-eGFP and histone H2A-mRFP show significant comobility, indicating that their motions are clearly interconnected in the nucleus, and 2) that the random motion of histones H2A within the chromatin network is subdiffusive, i.e., the effective diffusion coefficient decreases for slow timescales. Knocking out lamin A changes the diffusion back to normal. Thus, lamin A influences the dynamics of the entire chromatin network. Our conclusion is that lamin A plays a central role in determining the viscoelasticity of the chromatin network and helping to maintain local ordering of interphase chromosomes.

Citations

Jan 22, 2019·Genes, Chromosomes & Cancer·Andrew J FritzRonald Berezney
May 30, 2020·International Journal of Oncology·Daniel L PouliquenCatherine Guette
Dec 12, 2018·Genes, Chromosomes & Cancer·Anat VivanteYuval Garini
Aug 28, 2020·Cells·Alice-Anaïs VarletCatherine Badens
Dec 4, 2020·Experimental & Molecular Medicine·Yoori Kim, Hongtao Yu
Apr 19, 2020·Current Opinion in Genetics & Development·Maxime Mc TortoraDaniel Jost

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