Randomized controlled trial on the influence of dietary intervention on epigenetic mechanisms in children with cow's milk allergy: the EPICMA study

Scientific Reports
Lorella PaparoRoberto Berni Canani

Abstract

Epigenetic mechanisms could drive the disease course of cow's milk allergy (CMA) and formula choice could modulate these pathways. We compared the effect of two different dietary approaches on epigenetic mechanisms in CMA children. Randomized controlled trial on IgE-mediated CMA children receiving a 12-month treatment with extensively hydrolyzed casein formula containing the probiotic L.rhamnosus GG (EHCF + LGG) or with soy formula (SF). At the baseline, after 6 and 12 months of treatment FoxP3 methylation rate and its expression in CD4+ T cells were assessed. At same study points IL-4, IL-5, IL-10, and IFN-γ methylation rate, expression and serum concentration, miRNAs expression were also investigated. 20 children (10/group) were evaluated. Baseline demographic, clinical and epigenetic features were similar in the two study groups. At 6 and 12 months, EHCF + LGG group showed a significant increase in FoxP3 demethylation rate compared to SF group. At the same study points, EHCF + LGG group presented a higher increase in IL-4 and IL-5 and a higher reduction in IL-10 and IFN-γ DNA methylation rate compared to SF group. A different modulation of miR-155, -146a, -128 and -193a expression was observed in EHCF + LGG vs. SF. Dietary i...Continue Reading

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Citations

Mar 10, 2020·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Anna ForsbergMaria C Jenmalm
Apr 7, 2020·Current Opinion in Allergy and Clinical Immunology·Enza D'Auria, Carina Venter
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Aug 28, 2020·Frontiers in Pediatrics·Laura CarucciRoberto Berni Canani
May 24, 2020·International Journal of Environmental Research and Public Health·William B SherwoodFaisal I Rezwan
Dec 19, 2020·The Journal of Allergy and Clinical Immunology·Cheng-Jian XuGerard H Koppelman
Apr 22, 2021·Minerva Pediatrics·Dhinoth K BangarusamyAnnalisa Terranegra
Apr 13, 2021·Current Opinion in Allergy and Clinical Immunology·Maurizio MenniniAlessandro Giovanni Fiocchi
Jul 10, 2021·Pediatric Research·Jennifer Bermick, Matthew Schaller

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Methods Mentioned

BETA
fluorescence-activated cell sorting
ELISA
PCR
electrophoresis

Clinical Trials Mentioned

NCT02466035

Software Mentioned

Stata

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