RandomSpot: A web-based tool for systematic random sampling of virtual slides

Journal of Pathology Informatics
Alexander I WrightDarren Treanor

Abstract

This paper describes work presented at the Nordic Symposium on Digital Pathology 2014, Linköping, Sweden. Systematic random sampling (SRS) is a stereological tool, which provides a framework to quickly build an accurate estimation of the distribution of objects or classes within an image, whilst minimizing the number of observations required. RandomSpot is a web-based tool for SRS in stereology, which systematically places equidistant points within a given region of interest on a virtual slide. Each point can then be visually inspected by a pathologist in order to generate an unbiased sample of the distribution of classes within the tissue. Further measurements can then be derived from the distribution, such as the ratio of tumor to stroma. RandomSpot replicates the fundamental principle of traditional light microscope grid-shaped graticules, with the added benefits associated with virtual slides, such as facilitated collaboration and automated navigation between points. Once the sample points have been added to the region(s) of interest, users can download the annotations and view them locally using their virtual slide viewing software. Since its introduction, RandomSpot has been used extensively for international collaborativ...Continue Reading

References

Oct 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenB Pakkenberg
May 26, 1998·Journal of Neuropathology and Experimental Neurology·B T HymanM C Irizarry
Mar 10, 2015·Diseases of the Colon and Rectum·Eu-Wing TohPhilip Quirke

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Citations

Dec 18, 2015·BMC Cancer·Hannah PanayiotouMichele Cummings
Jun 26, 2015·Journal of Pathology Informatics·Alexander I WrightDarren E Treanor
Jan 12, 2017·Stem Cell Reports·Harry O KingSusan C Short
Feb 15, 2018·Journal of Clinical Pathology·Benjamin F DessauvagieRebecca Millican-Slater
May 11, 2018·Endocrine-related Cancer·Tom LeesValerie Speirs

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Software Mentioned

JQuery
MySQL
MATLAB
HTML5
RandomSpotDB
RandomSpot

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