Range of sizes of peptide products generated during degradation of different proteins by archaeal proteasomes.

The Journal of Biological Chemistry
A F KisselevA L Goldberg

Abstract

The 20 S proteasome processively degrades cell proteins to peptides. Information on the sizes and nature of these products is essential for understanding the proteasome's degradative mechanism, the subsequent steps in protein turnover, and major histocompatibility complex class I antigen presentation. Using proteasomes from Thermoplasma acidophilum and four unfolded polypeptides as substrates (insulin-like growth factor, lactalbumin, casein, and alkaline phosphatase, whose lengths range from 71 to 471 residues), we demonstrate that the number of cuts made in a polypeptide and the time needed to degrade it increase with length. The average size of peptides generated from these four polypeptides was 8 +/- 1 residues, but ranged from 6 to 10 residues, depending on the protein, as determined by two new independent methods. However, the individual peptide products ranged in length from approximately 3 to 30 residues, as demonstrated by mass spectrometry and size-exclusion chromatography. The sizes of individual peptides fit a log-normal distribution. No length was predominant, and more than half were shorter than 10 residues. Peptide abundance decreased with increasing length, and less than 10% exceeded 20 residues. These findings i...Continue Reading

References

Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Apr 28, 1995·Science·E SeemüllerW Baumeister
Mar 1, 1995·Nature Structural Biology·T Wenzel, W Baumeister
Oct 7, 1994·Cell·A Ciechanover
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·G NiedermannK Eichmann
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·A L GoldbergK L Rock
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·D StockJ Löwe
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Feb 1, 1997·Methods : a Companion to Methods in Enzymology·A Dey, K Ozato
Apr 3, 1997·Nature·D I Stuart, E Y Jones
Apr 1, 1997·Current Opinion in Structural Biology·W Baumeister, A Lupas

❮ Previous
Next ❯

Citations

Nov 30, 2000·Archives of Biochemistry and Biophysics·M Orlowski, S Wilk
Dec 22, 1999·Journal of Structural Biology·J WalzW Baumeister
Apr 6, 2006·Journal of Biosciences·Dipankar NandiDilip Chandu
Oct 1, 2006·Journal of Biological Physics·Alexey Zaikin, Juergen Kurths
Mar 8, 2005·Journal of Pediatric Surgery·David M OtterburnMarshall Z Schwartz
May 3, 2003·Biochemical and Biophysical Research Communications·Kei ShimizuKiyomi Kikugawa
Sep 11, 2002·Burns : Journal of the International Society for Burn Injuries·Jiake ChaiZhiyong Sheng
Feb 27, 1999·Current Opinion in Immunology·K Früh, Y Yang
May 14, 1999·Current Opinion in Immunology·S Uebel, R Tampé
Apr 8, 2000·Current Opinion in Structural Biology·P ZwicklA Steven
Nov 24, 2001·Surgical Oncology·S A ShahM P Callery
Oct 28, 1998·Trends in Cell Biology·D H Lee, A L Goldberg
Apr 16, 2003·Micron : the International Research and Review Journal for Microscopy·Antonella CecchettiniFranco Giorgi
Sep 2, 2003·Metabolic Engineering·Julie A Maupin-FurlowMalgorzata A Gil
Dec 22, 1999·The Biochemical Journal·M RechsteinerV Ustrell
Nov 7, 2001·Clinical and Experimental Dermatology·A Stolzing, T Grune
Jul 20, 1999·European Journal of Biochemistry·F GardratJ L Azanza
Oct 3, 2002·Journal of Neurochemistry·Della C DavidMaria Grazia Spillantini
Sep 21, 2012·The Journal of Biological Chemistry·Dara ForouzanAndrei N Lupas
Jul 12, 2008·The Journal of Biological Chemistry·Fernanda M CunhaEmer S Ferro
Mar 14, 2009·The Journal of Biological Chemistry·Denise A BertiLloyd D Fricker
Apr 14, 2009·The Journal of Biological Chemistry·Noa MedaliaAmi Navon
Feb 21, 2006·Antioxidants & Redox Signaling·Diana Poppek, Tilman Grune
May 29, 2002·The EMBO Journal·Paolo CascioAlfred L Goldberg
May 25, 2011·Journal of the National Cancer Institute·Amy M RuschakAaron D Schimmer
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P ZwicklW Baumeister
Oct 22, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Karin FeldererSusanne Witt
Mar 10, 2010·Biochemistry. Biokhimii︠a︡·A V SorokinL P Ovchinnikov
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M BochtlerR Huber
Jun 8, 1999·Annual Review of Immunology·K L Rock, A L Goldberg
Apr 5, 2005·Archaea : an International Microbiological Journal·Donald E WardRobert M Kelly
Apr 5, 2005·Archaea : an International Microbiological Journal·B FranzettiG Zaccai
Jan 5, 2014·Amino Acids·Sarina M HitzerdGodefridus J Peters
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A K NussbaumH Schild
Apr 12, 2014·Nature Communications·Zoe BudrikisStefano Zapperi
Jul 28, 2001·Physiologia Plantarum·Christina Ingvardsen, Bjarke Veierskov
Jun 28, 2014·Biomolecules·Paolo Cascio
May 13, 2010·Protein Expression and Purification·Semra KocabiyikSeda Ozdoğan
Sep 27, 2005·Immunological Reviews·Tom A M GroothuisJacques J Neefjes
Jun 4, 2004·The EMBO Journal·Silke HutschenreiterRobert Tampé

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.