PMID: 15223796Jun 30, 2004Paper

RANK, RANKL, OPG, and M-CSF expression in stromal cells during corneal wound healing

Investigative Ophthalmology & Visual Science
Steven E WilsonJuan P Rodriguez-Perez

Abstract

To examine the influx of monocytes into the cornea after epithelial scrape injury and the expression of chemokines that potentially regulate monocyte phenotype in cultured corneal fibroblasts and keratocytes in situ. Monocytes were detected by immunocytochemistry for the monocyte-specific antigen CD11b, in unwounded and epithelial scrape-wounded mouse corneas. The receptor activator of NF-kappa B ligand (RANKL), osteoprotegerin (OPG), and monocyte chemotactic and stimulating factor (M-CSF) mRNAs were detected in cultured mouse stromal fibroblasts by RT-PCR and RNase protection assay. RANKL, OPG, and M-CSF proteins were detected in cultured mouse stromal fibroblasts by immunoprecipitation and Western blot analysis. RANKL, RANK, M-CSF, and OPG proteins were detected in unwounded and wounded mouse corneas by immunocytochemistry. Chimeric mice with green fluorescent protein-labeled bone marrow-derived cells underwent corneal scrape injury and were monitored by fluorescence microscopy and immunocytochemistry. A small number of cells expressing the monocyte-specific CD11b antigen were detected in the stromas of unwounded mouse corneas. A larger number of CD11b-positive cells was detected in the stroma at 24 or 48 hours after epitheli...Continue Reading

Citations

Dec 14, 2011·Cornea·Marcony R SanthiagoSteven E Wilson
Oct 5, 2014·Experimental Eye Research·Andre A M Torricelli, Steven E Wilson
Jul 21, 2012·The Ocular Surface·Wanwen LanLouis Tong
Sep 22, 2011·Experimental Eye Research·M R SanthiagoS E Wilson
Aug 10, 2010·American Journal of Ophthalmology·Laura de Benito LlopisMiguel A Teus
Apr 27, 2010·Experimental Eye Research·Flavia L BarbosaSteven E Wilson
Oct 20, 2009·Journal of Refractive Surgery·Fabricio Witzel de MedeirosSteven E Wilson
Dec 18, 2015·Experimental Eye Research·Andre A M TorricelliSteven E Wilson
Jul 21, 2015·Experimental Eye Research·Boris Hinz
Mar 19, 2008·Experimental Eye Research·W Michael StapletonSteven E Wilson
Jul 28, 2007·Experimental Eye Research·Steven E WilsonFabricio W Medeiros
Dec 19, 2006·Bone·Philipp LeuchtJill A Helms
Aug 21, 2014·Journal of Orthopaedic Surgery and Research·Denglu YanYancheng Song
Jul 30, 2005·Stem Cells·Yiqin DuJames L Funderburgh
Apr 23, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tanya ZappitelliJane E Aubin
Jul 22, 2015·Progress in Retinal and Eye Research·Alexander V Ljubimov, Mehrnoosh Saghizadeh
Aug 23, 2005·Journal of Biomechanics·Jan G HazenbergDavid Taylor
Sep 15, 2005·International Journal of Pediatric Otorhinolaryngology·Giedre RaustyteJens Thomsen
May 18, 2013·Journal of Cataract and Refractive Surgery·Wolfgang J MayerDaniel Kook
Aug 14, 2013·PloS One·Krystel R HuxlinRichard P Phipps
Mar 23, 2007·Journal of Biomedical Materials Research. Part a·Hiroyuki KariyaYasuo Suda
Mar 21, 2019·Journal of Cellular and Molecular Medicine·Lingbo KongDingjun Hao
Nov 27, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rao MeFu-Shin X Yu
Jan 28, 2016·Clinical Science·Elsa C ChanGuei-Sheung Liu
Aug 30, 2018·Scientific Reports·Kye-Im JeonKrystel R Huxlin
Nov 7, 2019·Journal of Ophthalmology·Qiong Yi, Wen-Jin Zou
Dec 24, 2011·Journal of Strength and Conditioning Research·Andréia Silveira de SouzaLuiz Fernando Martins Kruel
Oct 18, 2020·Experimental Eye Research·Steven E WilsonRodrigo Carlos de Oliveira
Jun 20, 2020·Experimental Eye Research·Steven E Wilson
Oct 4, 2020·Experimental Eye Research·Steven E Wilson
Oct 16, 2020·Journal of Cellular and Molecular Medicine·Francesco PetrilloMichele D'Amico

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