RANKL Expression Is Increased in Circulating Mononuclear Cells of Patients with Calcific Aortic Stenosis

Journal of Cardiovascular Translational Research
Marcello RattazziPaolo Pauletto

Abstract

We aimed to investigate whether the expression of the OPG/RANK/RANKL triad in peripheral blood mononuclear cells (PBMC) and circulating levels of markers of ectopic mineralization (OPG, FGF-23, PPi) are modified in patients with calcific aortic valve disease (CAVD). We found that patients affected by CAVD (n = 50) had significantly higher circulating levels of OPG as compared to control individuals (p = 0.003). No differences between the two groups were found in FGF-23 and PPi levels. RANKL expression was higher in the PBMC from CAVD patients (p = 0.018) and was directly correlated with the amount of valve calcification (p = 0.032). In vitro studies showed that treatment of valve interstitial cells (VIC) with RANKL plus phosphate was followed by increase in matrix mineralization (p = 0.001). In conclusion, RANKL expression is increased in PBMC of patients with CAVD, is directly correlated with the degree of valve calcification, and promotes pro-calcific differentiation of VIC.

References

Jan 22, 2004·Journal of Molecular and Cellular Cardiology·Jens J KadenMartin Borggrefe
Jul 15, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Brian J BennettMichael E Rosenfeld
Oct 24, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Ping OlesenLars Melholt Rasmussen
Apr 18, 2008·Experimental and Molecular Pathology·Wei LiuManling Ma
Oct 4, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Marcello RattazziPaolo Pauletto
Jul 28, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·W Charles O'NeillChristopher W McIntyre
Aug 25, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lucia S GrahamRita B Effros
Jul 9, 2011·Journal of the American College of Cardiology·Jason P LinefskyBryan Kestenbaum
Nov 11, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Giacomo ZoppellaroMarcello Rattazzi
Apr 5, 2012·Nature Reviews. Endocrinology·Bithika Thompson, Dwight A Towler
Dec 12, 2012·Inflammation·Nancy CotéPatrick Mathieu
Mar 5, 2013·International Journal of Cardiology·Edit NagyMagnus Bäck
Jul 9, 2013·Circulation Research·Dwight A Towler
Oct 8, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Marcello RattazziPaolo Pauletto
Feb 19, 2014·Current Pharmaceutical Design·Giacomo BusoMarcello Rattazzi
Nov 8, 2015·European Heart Journal·Ricardo Villa-Bellosta, Jesús Egido
May 18, 2016·Nature Reviews. Disease Primers·Brian R LindmanPhilippe Pibarot
Nov 17, 2016·Bone·Shunsuke Yamada, Cecilia M Giachelli

❮ Previous
Next ❯

Citations

Nov 8, 2020·International Journal of Molecular Sciences·Maristella DonatoMarcello Rattazzi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.