RANTES is a pro-inflammatory chemokine and chemoattracts basophil cells to extravascular sites

The Journal of Pathology
P ContiT C Theoharides

Abstract

RANTES (regulated upon activation normal T expressed and secreted) is another member of the intercrine beta subfamily which acts as a selective chemoattractant for human monocytes and CD4-positive lymphocytes and increases the adherence of monocytes to endothelial cells. In this work, the effect of RANTES was studied on rat skin injection sites. Rats were intradermally injected with 50 microliters of RANTES, at different concentrations, fMet-Leu-Phe (FMLP), or LPS (positive controls) or PBS vehicle (negative control). The animals were then injected with 0.6 ml of Evans' blue in the tail vein in order to obtain a blue colour in the areas where the compounds were injected. After 4 h the rats were killed and the maximum diameter of the blue extravasation area was measured. The coloured areas were then excised and optical and electron microscopic studies were performed. In addition, in some of the excised tissue, a Northern blot analysis for histidine decarboxylase (HDC) mRNA was performed along with an estimation of the amount of histamine generated in the tissue injection sites. In these studies it was found that intradermal injections of 5, 2.5, and 1.25 x 10(-5) M RANTES produced a strong inflammatory response with the accumula...Continue Reading

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Citations

Dec 19, 1998·British Journal of Haematology·P ContiT C Theoharides
Nov 25, 2000·Journal of Hematotherapy & Stem Cell Research·A WallerL G Czaplewski
Mar 18, 2010·Expert Opinion on Biological Therapy·Natalia Lapteva, Xue F Huang
Dec 2, 2015·Geriatric Orthopaedic Surgery & Rehabilitation·Alasdair G SutherlandJanet Liversidge
Nov 6, 2007·Breast Cancer Research and Treatment·Mevan M JayasingheRobert A Kurt
Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P ContiT C Theoharides
Jan 10, 2006·The International Journal of Biological Markers·C TrakatelliJ Vecchiet
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A HeinemannI Sabroe
Oct 28, 2020·Acta Neurologica Scandinavica·Lukas HolmegaardChristina Jern

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