Raoultella bacteriophage RP180, a new member of the genus Kagunavirus, subfamily Guernseyvirinae

Archives of Virology
Mikhail V FofanovN Tikunova

Abstract

A novel lytic Raoultella phage, RP180, was isolated and characterized. The RP180 genome has 44,851 base pairs and contains 65 putative genes, 35 of them encoding proteins whose functions were predicted based on sequence similarity to known proteins. The RP180 genome possesses a gene synteny typical of members of the subfamily Guernseyvirinae. Phylogenetic analysis of the RP180 genome and similar phage genomes revealed that phage RP180 is the first member of the genus Kagunavirus, subfamily Guernseyvirinae, that is specific for Raoultella sp. The genome of RP180 encodes a putative protein with similarity to CRISPR-like Cas4 nucleases, which belong to the pfam12705/PDDEXK_1 family. Cas4-like proteins of this family have been shown to interfere with the bacterial host type II-C CRISPR-Cas system.

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Datasets Mentioned

BETA
MK737937

Software Mentioned

CGView
Vector NTI
PhyML3
BioEdit
BLASTx
SE
Unipro UGENE
tRNAscan
TBLASTX
SPAdes

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