Rap1 GTPase promotes coordinated collective cell migration in vivo

Molecular Biology of the Cell
Ketki SawantJocelyn A McDonald

Abstract

During development and in cancer, cells often move together in small to large collectives. To move as a unit, cells within collectives need to stay coupled together and coordinate their motility. How cell collectives remain interconnected and migratory, especially when moving through in vivo environments, is not well understood. The genetically tractable border cell group undergoes a highly polarized and cohesive cluster-type migration in the Drosophila ovary. Here we report that the small GTPase Rap1, through activation by PDZ-GEF, regulates border cell collective migration. We find that Rap1 maintains cell contacts within the cluster, at least in part by promoting the organized distribution of E-cadherin at specific cell-cell junctions. Rap1 also restricts migratory protrusions to the front of the border cell cluster and promotes the extension of protrusions with normal dynamics. Further, Rap1 is required in the outer migratory border cells but not in the central nonmigratory polar cells. Such cell specificity correlates well with the spatial distribution of the inhibitory Rapgap1 protein, which is higher in polar cells than in border cells. We propose that precisely regulated Rap1 activity reinforces connections between cell...Continue Reading

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Citations

May 1, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Maureen C LambTina L Tootle
Aug 9, 2020·Cancers·Ildefonso M De la Fuente, José I López
Aug 14, 2019·Current Opinion in Genetics & Development·Frank Macabenta, Angelike Stathopoulos
Jun 3, 2020·Molecular and Cellular Neurosciences·Yves Jossin

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Datasets Mentioned

BETA
GD14231

Methods Mentioned

BETA
GTPase
nucleotide exchange
two-hybrid
pull down
pull-down
GTPases
dissection
transgenic
PCR

Software Mentioned

Zeiss ZEN
ImageJ ( FIJI )
Excel
Zeiss AxioVision
GraphPad Prism
ImageJ

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