Rapamycin conditionally inhibits Hsp90 but not Hsp70 mRNA translation in Drosophila: implications for the mechanisms of Hsp mRNA translation.

Cell Stress & Chaperones
Roger F Duncan

Abstract

Rapamycin inhibits the activity of the target of rapamycin (TOR)-dependent signaling pathway, which has been characterized as one dedicated to translational regulation through modulating cap-dependent translation, involving eIF4E binding protein (eIF4E-BP) or 4E-BP. Results show that rapamycin strongly inhibits global translation in Drosophila cells. However, Hsp70 mRNA translation is virtually unaffected by rapamycin treatment, whereas Hsp90 mRNA translation is strongly inhibited, at normal growth temperature. Intriguingly, during heat shock Hsp90 mRNA becomes significantly less sensitive to rapamycin-mediated inhibition, suggesting the pathway for Hsp90 mRNA translation is altered during heat shock. Reporter mRNAs containing the Hsp90 or Hsp70 mRNAs' 5' untranslated region recapitulate these rapamycin-dependent translational characteristics, indicating this region regulates rapamycin-dependent translational sensitivity as well as heat shock preferential translation. Surprisingly, rapamycin-mediated inhibition of Hsp90 mRNA translation at normal growth temperature is not caused by 4E-BP-mediated inhibition of cap-dependent translation. Indeed, no evidence for rapamycin-mediated impaired eIF4E function is observed. These result...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Apr 15, 1974·Journal of Molecular Biology·A TissièresU M Tracy
Feb 25, 1980·Journal of Molecular Biology·S Lindquist
Jan 1, 1996·Molecular Biology of the Cell·N C BarbetM N Hall
Aug 23, 1996·Cell·E J Brown, S L Schreiber
Aug 29, 1997·The Journal of Biological Chemistry·A Haghighat, N Sonenberg
Jun 11, 1999·Genes & Development·A C GingrasN Sonenberg
Apr 12, 2001·Genes & Development·A C GingrasN Sonenberg
Nov 3, 2001·Genes & Development·A C GingrasN Sonenberg
Mar 16, 2002·Journal of Applied Physiology·Vladimir L Gabai, Michael Y Sherman
Jul 26, 2002·The Journal of Biological Chemistry·Andrew M BormanKatherine M Kean
Dec 4, 2003·Molecular and Cellular Biology·Mathieu MironNahum Sonenberg
Mar 17, 2006·The EMBO Journal·Thurl E HarrisJohn C Lawrence

❮ Previous
Next ❯

Citations

Oct 27, 2012·FEBS Letters·Mercedes de la FuenteJosé Luis Martínez-Guitarte
Jun 18, 2016·Viruses·Jason Liem, Jia Liu
Mar 1, 2019·Scientific Reports·Matthildi ValianouAristotelis Astrinidis
May 1, 2021·International Journal of Molecular Sciences·Rebeka PopovicL Miguel Martins
Jun 3, 2021·Insects·Palle Duun RohdeTorsten Nygaard Kristensen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.