Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins

Journal of Bioscience and Bioengineering
Tomonao Inobe, Nobuyuki Nukina

Abstract

Most proteins form larger protein complexes and perform multiple functions in the cell. Thus, artificial regulation of protein complex formation controls the cellular functions that involve protein complexes. Although several artificial dimerization systems have already been used for numerous applications in biomedical research, cellular protein complexes form not only simple dimers but also larger oligomers. In this study, we showed that fusion proteins comprising the induced heterodimer formation proteins FRB and FKBP formed various oligomers upon addition of rapamycin. By adjusting the configuration of fusion proteins, we succeeded in generating an inducible tetramer formation system. Proteins of interest also formed tetramers by fusing to the inducible tetramer formation system, which exhibits its utility in a broad range of biological applications.

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Citations

Nov 20, 2018·Microscopy·Marisa S Otegui, Jannice G Pennington
Nov 30, 2018·Molecular Biology of the Cell·David MelvilleRandy Schekman
May 24, 2019·The Journal of Physiology·Ji JingYubin Zhou
Dec 21, 2018·Frontiers in Pharmacology·Jürgen M KolosFelix Hausch
Jan 18, 2020·International Journal of Molecular Sciences·Cassandra M BarrettKarmella A Haynes
Aug 16, 2019·Disease Models & Mechanisms·Jonathan R VolpattiJames J Dowling
Dec 9, 2020·The FEBS Journal·Anže VerbičRoman Jerala
Mar 23, 2019·Current Opinion in Structural Biology·Maria Agustina Dominguez-Martin, Cheryl A Kerfeld
Aug 15, 2021·Nucleic Acids Research·Christopher M FurmanEric Alani

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