Rapamycin induced ultrastructural and molecular alterations in glomerular podocytes in healthy mice

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Kostas StylianouEugene Daphnis

Abstract

In the normal kidney, rapamycin is considered to be non-nephrotoxic. In the present study, we investigated whether rapamycin is indeed non-nephrotoxic by examining the ultrastructural and molecular alterations of podocytes in healthy mice. Balb/c mice were given three different intraperitoneal doses of rapamycin for 1 week (dose model)-low-dose group: 1 mg/kg/day, intermediate-dose (ID) group: 1.5 mg/kg/day and high-dose (HD) group: 3 mg/kg/day; four mice in each group. An ID of rapamycin was also given for three different periods (time model): 1, 4 and 8 weeks; four mice were in each group. Mice treated with dimethyl sulphoxide served as controls. Body weight was measured weekly. Renal function was assessed by serum creatinine at the time of sacrifice. For estimation of albuminuria, 24-h urine collections were performed before treatment and weekly thereafter. Glomerular content of nephrin, podocin, Akt and Ser473-phospho-Akt was estimated by western blot and immunofluorescence. Nephrin and podocin messenger RNA (mRNA) were measured by real-time polymerase chain reaction. Mean podocyte foot process width (FPW) was measured by electron microscopy. Urine albumin levels increased in the HD and 4-week groups. Renal function was mod...Continue Reading

References

Mar 1, 1992·Transplantation·J F DiJosephJ Y Chang
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·J F DiJosephS N Sehgal
Jan 1, 1993·Immunopharmacology and Immunotoxicology·J F Di Joseph, S N Sehgal
Oct 1, 1996·Kidney International·T F AndohW M Bennett
Aug 18, 2004·Genes & Development·Nissim Hay, Nahum Sonenberg
Feb 5, 2005·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Dirk R J Kuypers
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Mar 25, 2005·Journal of the American Society of Nephrology : JASN·Jian-Kang ChenRaymond C Harris
Jun 30, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Viorica BumbeaLionel Rostaing
Aug 10, 2005·American Journal of Nephrology·Finn Thomsen NielsenHans Dieperink
Nov 11, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Anna SaurinaFederico Oppenheimer
Nov 24, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C DanielC Hugo
Dec 24, 2005·Transplantation·Jacques J SennesaelGert A Verpooten
Apr 7, 2006·Journal of the American Society of Nephrology : JASN·Núria LloberasJosep M Grinyó
Apr 11, 2006·Molecular Cell·Dos D SarbassovDavid M Sabatini
May 30, 2006·Kidney International·M-J WuM-J Tang
Aug 17, 2006·Kidney International·J M van den AkkerA J Hoitsma
Sep 7, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Juan Carlos RuizFritz Diekmann
Aug 3, 2007·American Journal of Nephrology·Yi YangJianghua Chen
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Emmanuel LetavernierChristophe Legendre
Sep 7, 2007·Journal of the American Society of Nephrology : JASN·Fritz DiekmannJosep M Campistol
Sep 15, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Graeme RussDennis A Revicki
Nov 10, 2007·The American Journal of Pathology·Kavithalakshmi SataranatarajanBalakuntalam S Kasinath
Dec 21, 2007·American Journal of Physiology. Renal Physiology·Stephanie KrämerHarm Peters
Dec 21, 2007·The New England Journal of Medicine·Henrik EkbergUNKNOWN ELITE-Symphony Study
Feb 14, 2008·Nephron. Experimental Nephrology·Sandra WittmannChristian Hugo
May 1, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sing Leung LuiTak Mao Chan
May 16, 2008·Journal of the American Society of Nephrology : JASN·Kathrin HocheggerAlexander R Rosenkranz
Sep 25, 2008·Kidney International·Jeroen K J DeegensJack F M Wetzels
Oct 18, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Emmanuel LetavernierLaurent Baud
Oct 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andrew Y ChooJohn Blenis
Nov 5, 2008·Arthritis Research & Therapy·Padmalatha S ReddyMargot O'Toole
Nov 21, 2008·American Journal of Physiology. Renal Physiology·Beate VollenbrökerThomas Weide
Aug 17, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kostas StylianouEugene Daphnis

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Citations

Sep 27, 2013·Clinical & Developmental Immunology·Stavros StratakisEugene Daphnis
Jul 9, 2013·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Ioannis PetrakisEugene Daphnis
Aug 2, 2016·Nature Reviews. Nephrology·Daniel FantusAngus W Thomson
Oct 3, 2018·The Journal of Rheumatology·Desmond Y H YapTak Mao Chan
Mar 9, 2017·Journal of the American Society of Nephrology : JASN·Stefan ZschiedrichTobias B Huber
May 3, 2014·The Journal of Biological Chemistry·Diogo B PeruchettiWilliam B Guggino

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