Rapamycin inhibits human laryngotracheal stenosis-derived fibroblast proliferation, metabolism, and function in vitro

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
Daryan R NambaAlexander T Hillel

Abstract

To determine if rapamycin inhibits the growth, function, and metabolism of human laryngotracheal stenosis (LTS)-derived fibroblasts. Controlled in vitro study. Tertiary care hospital in a research university. Fibroblasts isolated from biopsies of 5 patients with laryngotracheal stenosis were cultured. Cell proliferation, histology, gene expression, and cellular metabolism of LTS-derived fibroblasts were assessed in 4 conditions: (1) fibroblast growth medium, (2) fibroblast growth medium with dimethylsulfoxide (DMSO), (3) fibroblast growth medium with 10(-10) M (low-dose) rapamycin dissolved in DMSO, and (4) fibroblast growth medium with 10(-9) M (high-dose) rapamycin dissolved in DMSO. The LTS fibroblast count and DNA concentration were reduced after treatment with high-dose rapamycin compared to DMSO (P = .0007) and normal (P = .0007) controls. Collagen I expression decreased after treatment with high-dose rapamycin versus control (P = .0051) and DMSO (P = .0093) controls. Maximal respiration decreased to 68.6 pMoles of oxygen/min/10 mg/protein from 96.9 for DMSO (P = .0002) and 97.0 for normal (P = .0022) controls. Adenosine triphosphate (ATP) production decreased to 66.8 pMoles from 88.1 for DMSO (P = .0006) and 83.3 for nor...Continue Reading

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Nov 3, 2016·Current Opinion in Otolaryngology & Head and Neck Surgery·David E Rosow, Eric Barbarite
Oct 16, 2015·The Annals of Otology, Rhinology, and Laryngology·Alexander T HillelUNKNOWN North American Airway Collaborative (NoAAC)
Mar 30, 2017·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Linda X YinAlexander T Hillel
Feb 6, 2018·The Laryngoscope·Adil AldhahraniChris Ward
Oct 17, 2018·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Robert J MorrisonAlexander Gelbard
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Nov 20, 2016·The Laryngoscope·Juan L Antón-PachecoJosé L Pablos
Aug 31, 2021·The International Journal of Biochemistry & Cell Biology·Chuin Ying UngTanya J Shaw

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