Rapamycin inhibits proliferation and differentiation of human endothelial progenitor cells in vitro

Experimental Cell Research
Martin ButzalWalter Fiedler

Abstract

Bone-marrow-derived, circulating endothelial precursor cells contribute to neoangiogenesis in various diseases. Rapamycin has recently been shown to have anti-angiogenic effects in an experimental tumor model. Our group has developed a culture system that allows expansion and endothelial differentiation of human CD133(+) precursor cells. We could show by PCR analysis that mTOR, the rapamycin-binding protein, was expressed in fresh CD133(+) cells, in expanded cells after 28 days, and in differentiated endothelial cells. Rapamycin inhibited proliferation of CD133(+) cells dose dependently at similar concentrations as hematopoietic Jurkat or HL-60 cells. Apoptosis was induced by rapamycin after 48 h of treatment, which could be reduced by preincubation with FK 506. Furthermore, the development of adherent endothelial cells from expanded CD133(+) cells was dose dependently inhibited. Expression of endothelial antigens CD144 and von Willebrand factor on differentiating endothelial precursors was reduced by rapamycin. In summary, rapamycin inhibits proliferation and differentiation of human endothelial precursor cells underlining its anti-angiogenic effects.

References

Jan 13, 1995·The Journal of Biological Chemistry·C J SabersR T Abraham
Apr 17, 1997·Nature·W Risau
Dec 15, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·M ToiK Kuroi
Jun 27, 2002·British Journal of Cancer·J HasanG C Jayson
Oct 3, 2002·Nature Reviews. Cancer·Napoleone Ferrara
Oct 18, 2002·Blood·Elvira PelosiCesare Peschle
Mar 13, 2003·Kidney International·Fu L LuanManikkam Suthanthiran
Apr 4, 2003·Advances in Experimental Medicine and Biology·Patrizia MancusoFrancesco Bertolini
Jun 10, 2004·Stem Cells and Development·Sonja LogesUrsula M Gehling

❮ Previous
Next ❯

Citations

Dec 3, 2013·Canadian Journal of Physiology and Pharmacology·Simon ChiangAdria Giacca
May 17, 2011·Journal of Cardiothoracic Surgery·Junjie ZouShui Wang
Jan 25, 2013·International Journal of Molecular Sciences·Zhi-Jun DaiWen-Ying Wu
Oct 13, 2006·Nature Reviews. Cancer·Francesco BertoliniRobert S Kerbel
Sep 11, 2012·Archives of Dermatological Research·Antonio Peramo, Cynthia L Marcelo
Apr 18, 2013·Cardiovascular Research·Kim Van der HeidenPaul C Evans
Sep 29, 2009·Investigational New Drugs·Alexander SchultzeWalter Fiedler
Aug 15, 2014·International Journal of Molecular Sciences·Fang TianFei Wang
Aug 23, 2006·The Journal of Cell Biology·Jiahu WangGordon W Laurie
Apr 23, 2008·Annals of Medicine·Jan SteffelFelix C Tanner
Oct 22, 2008·Transplantation Proceedings·S J N ChoiS Y Chung
Jul 24, 2007·Medical Hypotheses·Zheng Cao, Yujie Zhou
May 1, 2007·Biochemical and Biophysical Research Communications·Sonja LogesWalter Fiedler
Aug 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·María José SolerJosep Lloveras
Jun 27, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S G MiriukaT K Waddell
Sep 20, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·V CantaluppiG Camussi
Mar 14, 2009·Journal of Interventional Cardiology·Lakshmana PendyalaNicolas Chronos
Feb 16, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Aaron LiewTimothy O'Brien
May 30, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A S MühlfeldJ Floege
Oct 13, 2007·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Ryan BergFrank Litvack
Apr 6, 2011·Journal of Cellular Physiology·Li WangJunying Miao
Apr 23, 2014·Journal of Cellular Biochemistry·Xintong WangHak-Joon Sung
Jan 28, 2010·Transplant International : Official Journal of the European Society for Organ Transplantation·Chethan J SathyaVivek Rao
May 21, 2015·Journal of Interventional Cardiology·Feng ChenB O Feng
Feb 27, 2007·Journal of Hepatology·David SemelaJean-François Dufour
Feb 20, 2007·Journal of the American College of Cardiology·Eduard ShantsilaGregory Y H Lip
Feb 5, 2010·JACC. Cardiovascular Interventions·Gaku NakazawaRenu Virmani
Dec 3, 2014·Seminars in Immunology·Andrew H Moraco, Hardy Kornfeld
Apr 28, 2012·Neuroendocrinology·Jean-Yves Scoazec
Jan 7, 2009·Therapeutic Advances in Cardiovascular Disease·Barbara E StähliFelix C Tanner
Nov 19, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ayalew Tefferi
Mar 29, 2008·Gene Therapy·K-M DebatinC Beltinger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis