Rapeseed meal valorization strategies via nitrogen- and oxygen-limited production of polyhydroxyalkanoates with Pseudomonas putida

Waste Management
Phavit WongsirichotJames Winterburn

Abstract

Rapeseed meal (RSM) is a candidate for biopolymer production due to its abundance, low cost and potential integration with other rapeseed-derived products. However, existing studies pursuing such schemes are limited. The feasibility of different strategies for RSM valorization via protein extraction and polyhydroxyalkanoate production were evaluated. Nitrogen-limited RSM media was produced from hydrolysis of residues which had undergone extensive protein extraction using sodium hydroxide. A study of oxygen-limited fermentation was also performed on hydrolysate of untreated RSM via batch feeding. The typical strategy of using a high carbon-to-nitrogen ratio may not be the most suitable route for polyhydroxyalkanoate (PHA) production using nitrogen-rich biomass as a feedstock. Central composite design-based experiments show that due to mass transfer limitations protein extraction at 1-L scale could only achieve yields around 50% and 69%, at room temperature and 60 °C, respectively. Protein extraction yields reduced with successive extractions, meaning that whilst the RSM hydrolysate is viable for growth, designing a valorization scheme which has the fermentation step dictated by the protein extraction may not be practical/economi...Continue Reading

Citations

Jul 30, 2021·International Journal of Biological Macromolecules·Rajendran MuthurajTizazu H Mekonnen

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