Rapid 18O analysis of CO2 samples by continuous-flow isotope ratio mass spectrometry

Journal of Mass Spectrometry : JMS
D A Schoeller, A H Luke

Abstract

Continuous-flow isotope ratio mass spectrometry (CF-IRMS) has been shown to minimize sample preparation and reduce the sample size requirements and time for the determination of carbon and nitrogen. An inexpensive CF inlet was developed for the determination of the 18O isotopic abundance in CO2. Initial efforts were hampered by significant exchangeable oxygen within the inlet system, which was minimized by design changes and post-analysis mathematical correction. The system was tested for accuracy in the measurement of 18O abundance in standards and was accurate to 0.04/1000 with a precision of 0.03/1000. Additional tests were performed using urinary water for the measurement of energy expenditure by the doubly labeled water method and the results agreed to within 1% with those from traditional off-line, dual-inlet IRMS. The CF-IRMS reduced the analysis time to 2 min per sample compared with 15 min for traditional duel-inlet IRMS.

Citations

Mar 27, 2009·The American Journal of Clinical Nutrition·Jamie A CooperDale A Schoeller
Apr 24, 2009·The American Journal of Clinical Nutrition·Rajni SinghConnie M Weaver
Oct 27, 2004·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S B VotrubaD A Schoeller
May 29, 2012·Wilderness & Environmental Medicine·Walter S HailesBrent C Ruby
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Oct 4, 2014·Rapid Communications in Mass Spectrometry : RCM·Nicholas Paul Levitt
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Apr 19, 2017·Medicine and Science in Sports and Exercise·Andrea K ChomistekDavid R Bassett
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Feb 14, 2002·Journal of Applied Physiology·Stéphane BlancDale A Schoeller

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