Rapid, accurate, and sensitive fatty acid ethyl ester determination by gas chromatography-mass spectrometry

The Journal of Laboratory and Clinical Medicine
Clark C KuligGregory T Everson

Abstract

Fatty acid ethyl esters (FAEEs) are useful markers of ongoing alcohol use and may be associated with alcohol-induced damage to the liver and pancreas. In this article, we describe a novel method for rapid determination of the three major FAEEs found in human plasma. Internal standard, ethyl heptadecanoate, was added to plasma samples, and FAEEs were isolated by acetone precipitation, hexane lipid extraction, and amino-propyl silica solid phase extraction. FAEEs were quantitated by gas chromatography-mass spectrometry (GC-MS) using a nonpolar dimethylpolysiloxane column. The accuracy, precision, specificity, and sensitivity of the assay were defined from plasma samples from recently drinking and abstinent persons, with and without the addition of FAEEs. Individual FAEE peaks demonstrated excellent resolution. Instrument time was reduced by more than 60%. The lower limit of detection was 5 to 10 nM, and the lower limit of quantitation for each FAEE was 60 nM (for 22 samples with known concentration 60 nM, x +/-SD: 61 +/- 5.7, 57 +/- 5.7, and 57 +/- 5.9 nM, for ethyl palmitate, ethyl oleate, and ethyl stearate, respectively). Instrument precision (coefficient of variance, CV) for these three FAEEs was 0.3%, 0.4%, and 0.7%, respect...Continue Reading

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References

May 1, 1992·Pediatric Research·C F BearerC S Cook
Aug 1, 1983·The Journal of Clinical Investigation·L G Lange, B E Sobel
Feb 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M LaposataJ E Brown
Oct 9, 1996·JAMA : the Journal of the American Medical Association·K M DoyleM Laposata
Jan 26, 1996·Journal of Chromatography. B, Biomedical Applications·T G BernhardtM Laposata
Feb 4, 2003·Journal of Lipid Research·Catherine A BestMichael Laposata
Oct 23, 2003·The Journal of Pediatrics·Cynthia F BearerAndrew S Cwik
Jul 15, 2004·Alcoholism, Clinical and Experimental Research·Katrin BoruckiClaus Luley
Mar 31, 2005·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Adam BisagaSuzette M Evans
May 21, 2005·Alcohol·Bhupendra S KaphaliaG A S Ansari

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Citations

Jan 26, 2007·Alcoholism, Clinical and Experimental Research·John-Paul MorfinThomas Beresford
Feb 24, 2015·Alcoholism, Clinical and Experimental Research·Sowmya S MohanTheresa W Gauthier
Jun 20, 2006·Forensic Science International : Synergy·Fredrik C Kugelberg, Alan Wayne Jones
May 4, 2015·Analytical and Bioanalytical Chemistry·Pamela CabarcosAna María Bermejo
May 22, 2016·Clinical Biochemistry·Natalie KummerChristophe P Stove
Jun 8, 2017·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Clara Pérez-MañáRafael de la Torre
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Mar 11, 2010·Therapeutic Drug Monitoring·Carolyn M Zimmermann, Glen P Jackson

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