Rapid adaptive down regulation of oxidative burst induced by high dextrose in human umbilical vein endothelial cells

Diabetes Research and Clinical Practice
Mohamad H HoraniA D Mooradian

Abstract

To determine whether hyperglycemia-induced increase in oxidative burst undergoes adaptive changes, the time course of superoxide (SO) production in human umbilical vein endothelial cells treated with 13.75 mM (D250) or 27.5mM dextrose (D500) was measured using the hydroethidine (HE) fluorescence method. The rate of SO production (mean +/- S.D., in arbitrary units) in cells treated with D500 during the first hour (0.758 +/- 0.367) or with D250 (0.618 +/- 0.126) was significantly higher than the rate observed in control cells treated with 100mg/dl dextrose, (D100; 0.474 +/- 0.125) (P < 0.001). However, the rate of SO production during the second, third, fourth, and fifth hour was not significantly different from that measured in control cells. The fluorescence at baseline for control cells was 3.4 +/- 2.3 and for cells treated with D500 for 1, 2, 3, 4, and 5h was 3.4 +/- 1.9, 15.2 +/- 2.5, 21.6 +/- 2.3, 27.4 +/- 3.4, and 31.8 +/- 4.3 respectively (P < 0.001). The increased baseline fluorescence suggests that the antioxidant pool may be depleted within the first hour of exposure to high concentrations of dextrose. The latter possibility is supported by the observation that treatment of cells with varying concentrations of ascorbat...Continue Reading

References

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Nov 1, 1994·Metabolism: Clinical and Experimental·M P HabibA D Mooradian
May 1, 1994·Diabetes Care·A D MooradianJ Wylie-Rosett
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Feb 17, 1996·Lancet·P DandonaT Nicotera
Aug 1, 1997·Diabetic Medicine : a Journal of the British Diabetic Association·A Ceriello
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Feb 15, 2001·Archives of Internal Medicine·UNKNOWN DECODE Study Group, the European Diabetes Epidemiology Group.

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Citations

Nov 27, 2009·Diabetes Research and Clinical Practice·Mae Sheikh-AliArshag D Mooradian
Jan 19, 2010·Nutrition·Mae Sheikh-AliArshag D Mooradian
Aug 23, 2005·Diabetes/metabolism Research and Reviews
Oct 3, 2009·American Journal of Therapeutics·Mae Sheikh-AliArshag D Mooradian
Jan 24, 2006·American Journal of Therapeutics·Mohamad H HoraniArshag D Mooradian

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