Rapid analysis of acetone in human plasma by derivatization desorption electrospray ionization

The Analyst
Guangming XuShouzhuo Yao

Abstract

Desorption electrospray ionization mass spectrometry (DESI MS) is rapidly becoming a powerful analysis tool for a wide variety of samples in the open air. By combining DESI MS and derivatization, acetone in human blood samples, one of the diabetes mellitus (DM) biomarkers, was rapidly analyzed. Prior to DESI, the plasma sample was first exposed to hydroxylamine hydrochloride and acetone in plasma was derived into fixed oxime. The reactive products were validated by DESI/MS/MS. Quantification was performed in the positive-ion electrospray ionization mode by monitoring of the product ions at m/z 74 for acetone oxime and m/z 116 for 4-methyl-2-pentanone oxime as the internal standard. The limit of detection was of the order of magnitude of 0.10 ppbv and the linear range comprised two decades of concentration, covering the relevant concentration range of acetone in plasma samples. The method has achieved fast, high-throughput analysis of acetone in human plasma with little beforehand preparation and an experiment time of only a few seconds.

References

Apr 1, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·R HysplerV Balasová
Feb 24, 2001·Biosensors & Bioelectronics·Q ZhangX Gao
Nov 16, 2002·Analytical Chemistry·Heather LordJanusz Pawliszyn
Aug 16, 2003·Analytical Chemistry·Juan M Sanchez, Richard D Sacks
Sep 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chunhui DengXiangmin Zhang
Mar 1, 2005·Analytical Chemistry·Gary J Van BerkelMichael A Deibel
Apr 19, 2005·Chemical Communications : Chem Comm·Zoltán TakátsR Graham Cooks
Nov 22, 2005·Clinical Chemistry·Mark M KushnirA Wayne Meikle
Dec 29, 2005·Rapid Communications in Mass Spectrometry : RCM·Tiina J KauppilaRisto Kostiainen
Feb 1, 2006·Chemical Communications : Chem Comm·Hao ChenR Graham Cooks
Feb 24, 2006·Chemical Communications : Chem Comm·Ismael Cotte-RodríguezR Graham Cooks
Jun 1, 2006·Journal of the American Society for Mass Spectrometry·Marcela NefliuColin Moore
Jun 15, 2006·Rapid Communications in Mass Spectrometry : RCM·Tiina J KauppilaR Graham Cooks
Aug 5, 2006·Journal of Mass Spectrometry : JMS·Regina SparrapanRodinei Augusti
Apr 24, 2007·Journal of the American Society for Mass Spectrometry·Michael S Bereman, David C Muddiman

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