Rapid analysis of drug effects on the cell cycle

Cytometry
Z DarzynkiewiczM R Melamed

Abstract

Using a flow cytometric technique to analyse DNA content and chromatin structure simultaneously, the following parameters of cell cycle progression were estimated in control and drug-treated L1210 cell cultures: (a) the kinetics of cell exit from the G1 phase; (b) the probability of cell exit from the indeterminate portion of the G1 phase, measured as the half-time of cell residence in that state; (c) the duration of the deterministic portion of G1 phase; (d) the rates of cell transit through selected "windows" in S phase; (e) the rate of cell entrance into mitosis; (f) the mean duration of the cell cycle (Tc). These parameters are obtained in a single stathmokinetic experiment from measurements of individual samples withdrawn at 30 min-1 hr intervals from Vinblasatine-treated cultures. In the same experiment mitotic indices are obtained with high statistical accuracy, and may be used to determine the terminal point of drug action. In addition to cell cycle analysis the method makes it possible to detect drug-induced changes in nuclear chromatin that are manifested by varying sensitivity of DNA in situ to denaturation by acid. Such changes were found to be associated with defective chromatin condensation, altered histone modifi...Continue Reading

References

Apr 1, 1977·The Journal of Cell Biology·Z DarzynkiewiczM R Melamed
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z DarzynkiewiczM R Melamed
Jan 1, 1978·Journal of Cellular Physiology·B StorrieL Wenger
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A Smith, L Martin
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Sep 1, 1980·Cytometry·Z DarzynkiewiczM R Melamed

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Citations

Jan 1, 1993·Biology of the Cell·C Jayat, M H Ratinaud
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·T GoldkornC Chavez
Feb 1, 1986·The Journal of Clinical Investigation·L Staiano-CoicoJ M Hefton
Jan 1, 1984·Cancer Investigation·F Traganos
Jan 1, 1982·Progress in Histochemistry and Cytochemistry·R M Böhmer
Jun 23, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ralph M BöhmerLeonard C Harrison
Dec 1, 1981·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Apr 1, 1986·Journal of Cellular Physiology·C L MacLeodJ Mendelsohn
Nov 1, 1984·Cell and Tissue Kinetics·Z DarzynkiewiczM Kimmel
Sep 1, 1987·Experimental Cell Research·Z DarzynkiewiczP J Higgins

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