Rapid analysis of fentanyls and other novel psychoactive substances in substance use disorder patient urine using paper spray mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Joseph H KennedyJustin M Wiseman

Abstract

Drug overdose deaths due to fentanyls and other novel psychoactive substances (NPS) are on the rise. The higher potencies of fentanyl analogs compared with morphine require new technologies to identify and quantitate NPS. Paper spray tandem mass spectrometry (MS/MS) and high-resolution mass spectrometry were used to identify and measure fentanyl analogs as well as common drugs of abuse in urine samples from substance use disorder clinics. Ten-microliter urine samples were deposited directly on paper spray cartridges previously loaded with internal standards, dried, and analyzed with no other sample treatment. Quantitative results were obtained using MS/MS. Individual drugs were identified using high-resolution accurate mass spectrometry, and confirmed by data-dependent MS/MS. Calibration curves in urine were linear over a range of 0.5-50 ng/mL with R2 of 0.99 or better for eight representative fentanyl analogs. Cartridges preloaded with internal standards demonstrated satisfactory quantitative results compared with LC/MS. Direct identification and confirmation of fentanyl analogs and other common drugs of abuse in urine using high-resolution accurate mass and MS/MS fragmentation were demonstrated at low picogram levels. Paper s...Continue Reading

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Citations

Sep 19, 2018·Expert Review of Proteomics·Spencer ChiangZheng Ouyang
Feb 13, 2020·Mass Spectrometry Reviews·Scott A BordenPierangela Palma
Sep 7, 2019·Mass Spectrometry Reviews·Benjamin S FreyAbraham K Badu-Tawiah
Aug 1, 2020·Bioanalysis·Ana Luiza Freitas de Assis Linhares, Mauricio Yonamine
Jul 30, 2020·Mass Spectrometry Reviews·Kenyon Evans-NguyenChristopher C Mulligan
Aug 16, 2019·Rapid Communications in Mass Spectrometry : RCM·Catia CostaMelanie J Bailey
Aug 23, 2019·Journal of Mass Spectrometry : JMS·Gregory W Vandergrift, Chris G Gill
Oct 4, 2018·Journal of the American Society for Mass Spectrometry·Daniel O CarmanyTrevor Glaros
May 27, 2021·Mass Spectrometry Reviews·Maria Del Mar Boronat EnaVincenzo Abbate
Feb 23, 2019·Analytical Chemistry·Clara L FeiderLivia S Eberlin
Aug 31, 2021·Journal of the American Society for Mass Spectrometry·Henry WestGavin E Reid

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