Rapid analysis of sorbitol, galactitol, mannitol and myoinositol mixtures from biological sources

Journal of Chromatography
D M EadesW R Sherman

Abstract

The three commonly found hexitols mannitol, sorbitol and galactitol are well separated from each other and from myoinositol by gas chromatography as their butylboronate derivative on Dexsil-400, on a 1:1 mixture of OV-1 and OV-17, or on a DB-17 fused-silica capillary column. The method allows all four substances to be measured by autosampling electron ionization gas chromatography-mass spectrometry (GC-MS) in small tissue samples at organ concentrations as small as 5 mumol/kg wet mass in less than 4 min. Comparisons were made to determine the relative sensitivity of GC-MS and other detection methods. The order of sensitivity was electron ionization GC-MS greater than chemical ionization GC-MS greater than flame photometric detection using a boron-selective filter greater than hydrogen flame ionization detection.

Citations

Jan 1, 1991·The Journal of Clinical Investigation·B A WolfJ Turk
Dec 1, 1992·American Journal of Obstetrics and Gynecology·W R MeyerM P Diamond
Apr 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying DongJinglin Ding
Jan 5, 2002·Journal of Neuropathology and Experimental Neurology·R E SchmidtY Ido

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