Rapid analysis of two food-borne microbial communities at the species level by Fourier-transform infrared microspectroscopy

Environmental Microbiology
Mareike WenningSiegfried Scherer

Abstract

The species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. During recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. Fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. Infrared spectra of microcolonies of 70-250 microm in diameter can be recorded without producing a pure culture of the isolate. We have applied this novel technique for quantitative comparative analysis of two undefined, geographically separated food-borne smear cheese microbial consortia of limited complexity. Due to the high degree of automation, up to 200 microcolonies could be identified in 1 day and, in total, 3170 infrared spectra of microcolonies were recorded. The results obtained have been verified by Fourier-transform infrared macrospectroscopy and 16S rDNA sequencing. Interestingly, although the communities were unrelated, Staphylococcus equorum, Corynebacterium casei, Arthrobacter casei and Brevibacterium linens were found to ...Continue Reading

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Citations

Jan 31, 2008·Analytical and Bioanalytical Chemistry·Cecilia A Rebuffo-ScheerSiegfried Scherer
Jul 19, 2013·Applied Microbiology and Biotechnology·Mareike Wenning, Siegfried Scherer
Sep 14, 2013·The ISME Journal·Hannelore DanielThomas Clavel
Jan 17, 2012·Journal of Bacteriology·Christophe MonnetFrançoise Irlinger
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Jun 23, 2009·International Journal of Systematic and Evolutionary Microbiology·Thomas ClavelDirk Haller
Sep 15, 2009·International Journal of Systematic and Evolutionary Microbiology·Anne BleicherSiegfried Scherer
Jul 23, 2009·International Journal of Systematic and Evolutionary Microbiology·Verena S J SchmidtSiegfried Scherer
Mar 14, 2009·FEMS Yeast Research·Paolo RelliniGianluigi Cardinali
May 26, 2017·Critical Reviews in Analytical Chemistry·Nicoleta Elena DinaAlin Sebastian Porav
Jan 17, 2009·Applied Spectroscopy·Alan C SamuelsAshish Tripathi
Sep 2, 2016·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Evi Lang-HalterSiegfried Scherer
Nov 2, 2017·Biotechnology Journal·Cristina QuintelasClara Sousa
Jul 17, 2015·Foods·Mauricio I SantosAndrea Gomez-Zavaglia
Apr 30, 2017·Frontiers in Microbiology·Jesse P Harrison, David Berry

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